Effects of menstrual cycle phase on exercise-induced whole body DNA damage and new insights into molecular mechanisms of breast cancer development

本研究的目的是确定月经周期阶段对绝经前妇女进行2小时长时间骑车运动后氧化DNA损伤与乳腺癌之间关系的影响。12名娱乐活动频繁的痛经妇女作为研究对象。在60% vo2峰值时进行2小时的自行车运动前后，收集尿样以供后期尿液生物标志物分析。所有受试者在卵泡期和黄体期进行相同的运动方案。尿8-羟基-2′-脱氧鸟苷（8-OHdG， ng/mg肌酐）水平和雌激素代谢物（2-羟孕酮：2- ohe1 /16 α -羟孕酮，16 α - ohe1）比例的双向（时间x期）方差分析显示，主效应和相互作用均不显著。本研究的结果表明，月经周期阶段似乎不影响氧化DNA损伤和乳腺癌的发病率，绝经前妇女在急性中度运动2小时后。

The purpose of this study was to determine the effects of menstrual cycle phase on the relation between oxidative DNA damage and breast cancer following 2 h of prolonged cycling exercise in premenopausal women. Twelve recreationally active eumenorrheic women served as the subjects. Before and after 2 h of cycling exercise at 60%VO2peak, spot urine was collected for later analysis of urinary biomarkers. All subjects performed the same exercise protocol during the follicular and luteal phase. With regard to the levels of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG, ng/mg creatinine) and ratio of estrogen metabolites (2-hydroxyestrone: 2-OHE1/16alpha-hydroxyestrone, 16alpha-OHE1), a two-way (time x phase) ANOVA showed no significant main effects or interaction. The findings of the present study indicate that the menstrual cycle phase appears not to influence oxidative DNA damage and incidence of breast cancer in premenopausal women following 2 h of acute bouts of moderate exercise.