Regulation of the senescence responsive genes by nucleosomal change

在这项研究中，我们验证了5-溴脱氧尿苷是否可以改变核小体结构的派-1基因的启动子区迅速响应5-溴脱氧尿苷在人HeLa细胞。我们发展了“引物延伸法”来精确分析核小体在DNA上的定位。用微球菌核酸酶消化染色质DNA，然后用特异性引物进行接头连接和PCR，显示了被认为来自核酸酶消化的特异性条带。通过消除非特异性条带的努力，我们达到了一种可以精确检测核小体结构变化的情况。

In this study, we verified whether 5-bromodeoxyuridine can change nucleosomal structure in the promoter region of the PAI-1 gene quickly responsive to 5-bromodeoxyuridine in human HeLa cells. We developed "the primer extension method" to precisely analyze the positioning of nucleosomes on DNA. Digestion of the chromatin DNA with micrococcal nuclease followed by linker ligation and PCR with specific primers revealed specific bands that are thought to be derived from digestion with the nuclease. With efforts to eliminate non-specific bands, we came to a situation where we can precisely detect a change in the nucleosommal structure.