Identification of novel IAA transport inhibitors and their functions

以玉米胚芽组织为材料，建立了两种化学筛选方法，分别对籽粒向地曲率和籽粒IAA量的影响进行了评价。利用HitFinder 10000个化学文库，鉴定出8个IAA外排抑制剂候选化合物，发现其中4个化合物具有致死性并影响PIN蛋白的细胞转运。我们还发现10种化合物即使在较高浓度下也能轻微影响IAA在玉米胚芽组织中的转运。其中一种化合物与合成生长素2,4- d相似，可能优先抑制IAA内流。这些活性化合物可能为研究IAA转运机制和鉴定参与IAA转运系统的新蛋白组分提供新的工具。

We established two chemical screening methods by assessing the effect on gravitropic curvature and on IAA quantity transported from tip sections using the maize coleoptile systems. We used HitFinder 10,000 chemical library and identified 8 candidate compounds of IAA efflux inhibitor and it was revealed that 4 compounds among them showed lethality and affected cellular trafficking of PIN protein. We also found 10 compounds which affected slightly IAA transport in maize coleoptiles even at higher concentrations. One of the compound was similar to that of synthetic auxin 2,4-D, and it is possible that it could preferentially inhibit IAA influx. These active compounds may represent new tools for dissecting mechanisms of IAA transport and for identifying new protein components involving in IAA transport systems.