Identification of novel IAA transport inhibitors and their functions

我们建立了两种化学筛选方法，通过评估玉米胚芽鞘系统对向心曲率的影响和对顶端切片运输的IAA数量的影响。我们利用HitFinder 10000化学文库，鉴定了8个IAA外排抑制候选化合物，发现其中4个化合物具有致死性，并影响PIN蛋白的细胞转运。我们还发现了10种化合物，它们即使在较高浓度下也会对玉米胚芽鞘中的IAA运输产生轻微的影响。其中一个化合物类似于合成的生长素2，4-D，可能具有优先抑制IAA内流的作用。这些活性化合物可能是解剖IAA转运机制和鉴定涉及IAA转运系统的新的蛋白质组分的新工具。

We established two chemical screening methods by assessing the effect on gravitropic curvature and on IAA quantity transported from tip sections using the maize coleoptile systems. We used HitFinder 10,000 chemical library and identified 8 candidate compounds of IAA efflux inhibitor and it was revealed that 4 compounds among them showed lethality and affected cellular trafficking of PIN protein. We also found 10 compounds which affected slightly IAA transport in maize coleoptiles even at higher concentrations. One of the compound was similar to that of synthetic auxin 2,4-D, and it is possible that it could preferentially inhibit IAA influx. These active compounds may represent new tools for dissecting mechanisms of IAA transport and for identifying new protein components involving in IAA transport systems.