Identification of a plant molecule involved in a surveillance system for the symbiotic nitrogen fixation and its localization in the symbiotic tissues

共生固氮监测系统中涉及的植物分子的鉴定及其在共生组织中的定位

• 批准号: 23570058
• 负责人: UCHIUMI Toshiki
• 金额: $ 3.41万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570058/
• 关键词: 植物微生物相互作用・共生; ミヤコグサ; 根粒菌; 共生窒素固定; リポ多糖; リポ多糖結合タンパク質; 形質転換; 毛状根

Lipopolysaccharide (LPS) of bacteria induces the plant resistance in pathogenic combination and is also involved in the establishment of symbiosis between legumes and rhizobia. However, LPS recognition system is still unknown in plants. Referring LBP of mammals, four LBP-candidates were identified on the genome of Lotus japonicus and referred to as LjLBPs. N-terminal barrel of LjLBPs bound with LPS of Escherichia coli. Hairy roots transformed with the fusion genes of LjLBPs and mOrange revealed the localization of LjLBPs. LjLBPs were secreted to the outside of the roots and, in the nodule, targeted to the rhizobia that penetrated into the host plant cells. We investigated the symbiotic phenotype of the hairy roots carrying RNAi- and overexpressing- constructs, and mutant lines generated by EMS treatment or LORE1 insertion. However, we could not establish the idea that LjLBPs were essential for the symbiosis between L. japonicus and its microsymbiont Mesorhizobium loti.

细菌脂多糖可诱导植物对病原菌的抗性，也参与了豆科植物与根瘤菌之间共生关系的建立。然而，植物中的内毒素识别系统尚不清楚。参考哺乳动物的LBP，在莲花基因组上鉴定出四个LBP候选基因，称为LjLBPs。LjLBPs的N端桶与大肠杆菌的内毒素结合。用LjLBPs和Morange融合基因转化毛状根，发现了LjLBPs的定位。LjLBPs被分泌到根的外部，在根瘤中，针对渗透到寄主植物细胞的根瘤菌。我们研究了携带RNAi和过表达构建体的毛状根以及由EMS处理或LORE1插入产生的突变系的共生表型。然而，我们不能确定LjLBPs对于日本血吸虫与其共生菌中的共生菌loti之间的共生是必不可少的。

项目成果

根粒着生オートレギュレーションとβ-1,3- glucanase遺伝子(LjGlu1)の発現

结瘤自动调节和β-1,3-葡聚糖酶基因（LjGlu1）的表达

• 发表时间: 2012
• 作者: 蘭正人;小薄健一;鈴木章弘;原仁俊;山下健司;石原真美;小林優子;浅見忠男;九町健一;東四郎;阿部美紀子;内海俊樹
• 通讯作者: 内海俊樹

Lipopolysaccharide binding protein, a new requirement for legume-rhizobium symbiosis

脂多糖结合蛋白，豆科植物-根瘤菌共生的新要求

• 发表时间: 2011
• 作者: Murakami E;Takayama H;Osuki K;Nagata M;Hori T;Shigeoka M;Sahara M;Muto S;Nagano Y;Kucho K;Abe M;Sato S;Higashi S;Uchiumi T.
• 通讯作者: Uchiumi T.

Characterization of nitric oxide-inducing lipid A derived from Mesorhizobium loti lipopolysaccharide.

• DOI: 10.1264/jsme2.me12103
• 发表时间: 2012
• 期刊: Microbes and environments
• 影响因子: 2.2
• 作者: Hashimoto M;Tanishita Y;Suda Y;Murakami E;Nagata M;Kucho K;Abe M;Uchiumi T
• 通讯作者: Uchiumi T

Class 1 ヘモグロビンの変異はMesorhizobium lotiの宿主植物Lotus japonicusへの感染に影響する

1类血红蛋白突变影响中索根瘤菌对寄主植物百脉根的感染

• 发表时间: 2012
• 作者: 角友博;小薄健一;九町健一;阿部美紀子;東四郎;内海俊樹
• 通讯作者: 内海俊樹

Cysteine rich peptides as host-derived molecules that regulate microsymbionts

富含半胱氨酸的肽作为调节微共生体的宿主衍生分子

• 发表时间: 2013
• 作者: Hashimoto M;Mizukami M;Suda Y;Kucho K;Abe M;Uchiumi T.;Uchiumi T.
• 通讯作者: Uchiumi T.