Analysis of mitochondrial morphology and movement

线粒体形态和运动分析

• 批准号: 23570232
• 负责人: OKA Toshihiko
• 金额: $ 3.49万
• 依托单位: Rikkyo University (2012-2013)Kyushu University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570232/
• 关键词: ミトコンドリア; オルガネラ

An RNA interference screen using nematode led us to identification of a novel kinasin KLP6 involved in mitochondrial morphology and movement. The dominant-negative KLP6 induced abnormal mitochondrial morphology in HeLa cells. Furthermore, anterograde transport of mitochondria in the neurites of differentiated neuro2a cells was significantly reduced when the dominant negative form was overexpressed, indicating that KLP6 regulates intracellular transport of mitochondria.Downregulation of an inner membrane protein LETM1 induces mitochondrial swelling and fragmentation. Moreover, cristae structure clearly disappeared in the LETM1-knockdown cells. To investigate the LETM1 functions, we attempted to in vitro reconstitute membrane invagination using LETM1 recombinant protein and artificial liposome. This is a good tool to analyze the roles of LETM1 on the formation of mitochondrial inner membrane structures.

利用线虫的RNA干扰筛选使我们鉴定出一种参与线粒体形态和运动的新型激酶KLP6。显性阴性KLP6诱导HeLa细胞线粒体形态异常。此外，当显性阴性形式过表达时，分化的neuro2a细胞的神经突中线粒体的顺行运输显著减少，这表明KLP6调节线粒体的细胞内运输。细胞膜蛋白LETM1下调可诱导线粒体肿胀和断裂。此外，在letm1敲低的细胞中，嵴结构明显消失。为了研究LETM1的功能，我们尝试用LETM1重组蛋白和人工脂质体体外重建膜内陷。这是分析LETM1在线粒体内膜结构形成中的作用的一个很好的工具。

项目成果

KLP6, a newly identified kinesin that regulates morphology and transport of mitochondria in neuronal cells.

KLP6 是一种新发现的驱动蛋白，可调节神经元细胞中线粒体的形态和运输。

• 发表时间: 2011
• 期刊: Journal of Cell Science
• 影响因子: 4
• 作者: Tanaka;K.;Sugiura;Y.;Ichishita;R.;Mihara;K.;and Oka;T
• 通讯作者: T

KLP6, a newly identified kinesin that regulates morphology and transport of mitochondria in neuronal cells

KLP6，一种新发现的驱动蛋白，可调节神经元细胞中线粒体的形态和运输

• 发表时间: 2011
• 期刊: J. Cell Sci.
• 作者: Tanaka;K.;Y. Sugiura;R. Ichishita;K. Mihara and T. Oka.
• 通讯作者: K. Mihara and T. Oka.

Formation of Cristae Structure in Mammals

哺乳动物嵴结构的形成

• 发表时间: 2013
• 作者: Hiroto Mizushima;Mitsuki Funakoshi;Eisuke Mekada;Toshihiko Oka.
• 通讯作者: Toshihiko Oka.

A Dimeric PINK1-containing Complex on Depolarized Mitochondria Stimulates Parkin Recruitment

• DOI: 10.1074/jbc.m113.509653
• 发表时间: 2013-12-20
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Okatsu, Kei;Uno, Midori;Matsuda, Noriyuki
• 通讯作者: Matsuda, Noriyuki

オルガネラ形態の機能的意義

细胞器形态的功能意义

• 发表时间: 2014
• 作者: Hiroto Mizushima;Mitsuki Funakoshi;Eisuke Mekada;岡敏彦
• 通讯作者: 岡敏彦