Study on yeast mRNA flux in ethanol stress response and during the process of alcoholic fermentation

我们发现，不必要的mRNA是不翻译和分离的P体或应力颗粒（SG）在酵母细胞在酒精发酵的应力条件下。我们还展示了几个数据，表明P-体和SGs的贡献，有效和选择性的翻译的mRNA，真正需要的快速应激反应。此外，我们分离和表征了几种在乙醇胁迫条件下优先翻译的mRNA。此外，我们证实，乳酸可以诱导P-体的形成和翻译抑制，但没有SG的形成。乙酸和丙酸对mRNP颗粒形成的影响与乳酸不同。

We found that unnecessary mRNA is not translated and segregated in P-bodies or stress granules (SGs) in yeast cells under stress conditions of alcoholic fermentation. We also demonstrated several data that indicate the contribution of P-bodies and SGs to effective and selective translation of mRNAs that are really required for the rapid stress response. Additionally, we isolated and characterized several mRNA that were preferentially translated under ethanol stress conditions. Furthermore, we verified that lactic acid can induce P-body formation and translational repression but not SG formation. Effects of acetic acid and propionic acid on mRNP granule formation were different from that of lactic acid.