Continuous luminescence of photoprotein with substrate analog

人们试图找到使光蛋白连续发光的条件。利用化学合成的氧-发色团类似物获得了光蛋白再生的适宜条件。合成了一种光蛋白荧光物质。最后合成了一种含有硫原子和氟原子的类似物质。该类似物与天然物质一样具有较高的生物发光活性。从这些结果中积累了获得光蛋白连续发光的基础知识。

Attempts have been made to find the condition which enables photoprotein to emit light continuously. A suitable condition for re-generation of photoprotein was obtained by using chemically synthesized oxy-chromophore analog. A florescent substance of photoprotein was also synthesized. A substance analog, in which sulfur and fluorine atoms were incorporated, was finally synthesized. The analog showed high bioluminescent activity as natural substance. From these results, fundamental knowledge was accumulated to obtain continuous luminescence of photoprotein.