Study on the regulatory mechanisms of neurodegenelation by tryptophan key enzymes

色氨酸关键酶调控神经退行性变机制的研究

• 批准号: 23580158
• 负责人: EGASHIRA Yukari
• 金额: $ 3.58万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580158/
• 关键词: トリプトファン; ナイアシン; キノリン酸; 神経毒; ミクログリア; 脳; 炎症; ACMSD; NAD; IDO; SREBP; LPS

Quinolinic acid (QA), a tryptophan metabolite, was observed to increase in brains of patients with neurodegenerative diseases. Indoleamine 2,3-deoxygenase (IDO) and amino carboxymuconate semialdehyde decarboxylase (ACMSD) affect QA production. In this study, we investigated the effect of some food components on IDO or ACMSD expression in microglial cells or LPS treated mice involved in neurodegenerative disease pathogenesis. Polyphenols in cereals suppressed IDO expression in microglial cells and in brain of LPS treated mice. Our results suggest that inhibition of the NF-kB pathway may be partly involved in the regulatory mechanism of IDO expression by polyphenols in cereals. We also found that phytol decreased ACMSD activity, its protein and mRNA expression. It is possible that this mechanism resulted from the activation of PPAR alpha. Some food components may be considered helpful for avoiding central nervous system inflammation.

喹啉酸（QA），色氨酸代谢产物，被观察到增加在大脑中的神经退行性疾病患者。吲哚胺2，3-脱氧酶（IDO）和氨基羧基粘康酸半醛脱羧酶（ACMSD）影响QA的产生。在这项研究中，我们研究了一些食物成分对IDO或ACMSD在小胶质细胞或LPS处理的参与神经退行性疾病发病机制的小鼠中表达的影响。谷物中的多酚抑制小胶质细胞和LPS处理的小鼠脑中的IDO表达。我们的研究结果表明，抑制NF-κ B途径可能部分参与了调节机制的IDO表达的多酚类物质在谷物。我们还发现，植醇降低ACMSD活性，其蛋白和mRNA的表达。这种机制可能是由于激活了PPAR α。一些食物成分可能被认为有助于避免中枢神经系统炎症。

项目成果

Effect of dietary phytol on the expression ofα-amino-β- carboxymuconate-ε-semialdehyde decarboxylase, a key enzyme of tryptophan-niacin metabolism in rats

膳食植醇对大鼠色氨酸-烟酸代谢关键酶α-氨基-β-羧基粘康酸-ε-半醛脱羧酶表达的影响

• 发表时间: 2012
• 作者: Hiroko Matsuda;Ryo-ta Gomi;Shizuka Hirai;Yukari Egashira
• 通讯作者: Yukari Egashira

Effect of dietary phytol on the expression of α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase, a key enzyme of tryptophan-niacin metabolism in rats

膳食植醇对大鼠色氨酸-烟酸代谢关键酶α-氨基-β-羧基粘康酸-ε-半醛脱羧酶表达的影响

• 发表时间: 2013
• 期刊: Bioscience, Biotechnology and Biochemistry
• 作者: Hiroko Matsuda;Ryo-ta Gomi;Shizuka Hirai;Yukari Egashira
• 通讯作者: Yukari Egashira

Modulatory effect of ferulic acid on LPS-induced indoleamine 2, 3-deoxygenase (IDO) expression in microglia.

阿魏酸对 LPS 诱导的小胶质细胞吲哚胺 2, 3-脱氧酶 (IDO) 表达的调节作用。

• 发表时间: 2013
• 作者: M Koshiguch;H Komazaki;M Otsuka;S Hirai;Y Egashira
• 通讯作者: Y Egashira

A mechanism for the production of quinolinic acid by dietary components or medicine

通过膳食成分或药物产生喹啉酸的机制

• 发表时间: 2012
• 作者: Yukari Egashira;Naho Sasaki;Kuniaki Saito,Minori Iijima
• 通讯作者: Kuniaki Saito,Minori Iijima

Regulation of rat hepatic α-amino-β-carboxymuconate-ε-semialdehyde decarboxylase, a key enzyme in the tryptophan-NAD pathway, by dietary cholesterol and sterol regulatory element-binding protein-2

• DOI: 10.1007/s00394-013-0547-1
• 发表时间: 2014-03-01
• 期刊: EUROPEAN JOURNAL OF NUTRITION
• 影响因子: 5
• 作者: Matsuda, Hiroko;Sato, Mayumi;Egashira, Yukari
• 通讯作者: Egashira, Yukari