Search of the new anticancer drug material which makes tublin a molecular target from the marine invertebrate as

在本应用研究中，为了开发海洋无脊椎动物微管蛋白聚合和解聚抑制物质作为抗肿瘤新药的种子化合物，并分离有效成分，采集了海洋无脊椎动物，对每种动物进行了加工，得到了粗提物。在此基础上，首次找到了在神经生长因子存在下可能对PC12细胞的作用以及对微管蛋白功能有影响的提取物。通过对海绵、海参和从中华布格拉菌进一步分离得到的细菌培养提取物的活性成分进行了检索，认为该活性物质将被展示出来。分离了神经酰胺、脑苷脂和二酮基哌嗪衍生物。为了评估结构活性关系所需的几种模拟对象的合成也开始了。

In this application research, in order to develop the tubulin polymerization and the depolymerization inhibitory substance of the marine invertebrate as a seed compound of a new antineoplastic drug and to separate an active ingredient, the sea invertebrate animal was collected, each animal was processed, and the rough extract was obtained. Furthermore, the extract which has a possibility of having investigated the action on PC12 cell and having affected the tubulin function under nerve growth factor existence about the obtained sample as the first activity testing was found out. The active ingredient was searched about the sponges, sea cucumbers, and the cultivation extract of the bacteria further separated from Bugula neritina, that was accepted to be that activity will be shown. A ceramide, cerebrosides, and diketopiperazine derivatives have been separated. Synthesis of several sorts of analog objects, required in order to evaluate structure activity relationship, was also started.