Metabolite profiling of biological steroids in pathological tissue specimen

病理组织标本中生物类固醇的代谢谱

• 批准号: 23590051
• 负责人: HONDA Akira
• 金额: $ 2.41万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590051/
• 关键词: ステロイド; LC-MS/MS

We have developed a highly sensitive and specific method for the analysis of steroid profiles in biological samples including pathological tissue specimens. After the addition of deuterium-labeled internal standards, biological samples were saponified with 1N ethanolic KOH, extracted with n-hexane followed by n-hexane:ethylacetate (1:1), and derivatized into picolinyl esters before analysis by high performance liquid chromatography-positive electrospray ionization-tandem mass spectrometry (HPLC-PESI-MS/MS). Cholesterol related sterols, oxysterols, adrenal steroids and neurosteroids were quantified simultaneously. The effects of formaldehyde fixation and Mayer's hematoxylin staining on the steroid analysis of pathological tissue specimen were examined. These procedures did not affect significantly the results of the present analysis.

我们已经开发了一种高度敏感和特异性的方法来分析生物样品中的类固醇谱，包括病理组织标本。生物样品加入氘标记内标后，用1N乙醇KOH皂化，用正己烷-正己烷：乙酸乙酯（1:1）萃取，衍生成吡啶酯，然后用高效液相色谱-正电喷雾-串联质谱（HPLC-PESI-MS/MS）分析。同时定量测定胆固醇相关固醇、氧甾醇、肾上腺类固醇和神经类固醇。检测甲醛固定和Mayer氏苏木精染色对病理组织标本类固醇分析的影响。这些程序对本分析的结果没有显著影响。

项目成果

加齢と生活習慣病によるコレステロール(CHOL)代謝の変化

由于衰老和生活方式相关疾病导致的胆固醇 (CHOL) 代谢变化

• 发表时间: 2013
• 作者: Otsuka;T.;Iwao;Y.;Miyagishima;A.;Itai;S.;本多彰,池上正,宮﨑照雄,松﨑靖司
• 通讯作者: 本多彰,池上正,宮﨑照雄,松﨑靖司

NAFLD患者血清における酸化ステロール上昇とその意義

NAFLD患者血清中甾醇氧化物升高及其意义

• 发表时间: 2012
• 作者: Agata;Y.;Iwao;Y.;Shiino;K.;Miyagishima;A.;Itai;S.;池上正,本多彰,松﨑靖司
• 通讯作者: 池上正,本多彰,松﨑靖司

アルコール性脂肪肝患者の血清脂質メタボローム解析

酒精性脂肪肝患者血清脂质代谢组分析

• 发表时间: 2012
• 作者: Agata Y;Iwao Y;Shiino K;Miyagishima A;Itai S.;本多彰,松﨑靖司
• 通讯作者: 本多彰,松﨑靖司

Combination therapy of primary biliary cirrhosis with ursodeoxycholic acid and a dual PPARα/PXR agonist, bezafibrate

熊去氧胆酸和 PPARα/PXR 双重激动剂苯扎贝特联合治疗原发性胆汁性肝硬化

• 发表时间: 2012
• 作者: Honda A;Ikegami T;Miyazaki T;Nakamuta M;Takikawa H;Matsuzaki Y.
• 通讯作者: Matsuzaki Y.

Cholesterol 25-hydroxylation activity of CYP3A

• DOI: 10.1194/jlr.m014084
• 发表时间: 2011-08-01
• 期刊: JOURNAL OF LIPID RESEARCH
• 影响因子: 6.5
• 作者: Honda, Akira;Miyazaki, Teruo;Matsuzaki, Yasushi
• 通讯作者: Matsuzaki, Yasushi