Interaction of WNT family and TGF-beta family during myogenic differentiation of mesenchymal progenitor cells

WNT家族和TGF-β家族在间充质祖细胞生肌分化过程中的相互作用

• 批准号: 23590227
• 负责人: NOHNO Tsutomu
• 金额: $ 3.33万
• 依托单位: Kawasaki Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590227/
• 关键词: WNT; TGFβ; 骨格筋分化; マイオスタチン; BMP4; βカテニン; C2C12; 骨格筋; 筋分化; miRNA; Smad1/5; 筋芽細胞; Wnt3a; Wnt4; noggin; ウイルスベクター

Several Wnt signaling components including Wnt4, Sfrp2, and Porcupine were induced after serum starvation during myogenic differentiation in C2C12 myoblast cell line, and Wnt4 was most effective to induce myogenic differentiation through suppression of beta-catenin signaling with Wnt3a, when overexpressed in C2C12 cells. Stable Wnt4-expressing sub-cell lines (W4-08) of C2C12 indicate decreased proliferation accompanying spontaneous differentiation into myotube cells with myosin heavy chain expression. BMP4 expression was up-regulated in W4-08 cells, as observed in C2C12 cells after serum starvation. BMP4 is acting to down-regulate myogenic differentiation, and noggin is effective to promote myogenic differentiation in the presence of Wnt3a and Wnt4, suggesting possible interaction between Wnt/beta-catenin and BMP/Smad pathways.

在C2 C12成肌细胞系中，在血清饥饿之后，包括Wnt 4、Sfrp 2和Porcupine在内的几种Wnt信号传导组分被诱导成肌分化，并且当Wnt 4在C2 C12细胞中过表达时，Wnt 3 a通过抑制β-catenin信号传导而最有效地诱导成肌分化。C2 C12的稳定Wnt 4表达亚细胞系（W 4 -08）表明增殖降低，伴随自发分化成具有肌球蛋白重链表达的肌管细胞。在W 4 -08细胞中，BMP 4表达上调，与血清饥饿后在C2 C12细胞中观察到的一样。BMP 4下调肌原性分化，而头蛋白在Wnt 3a和Wnt 4存在下有效促进肌原性分化，表明Wnt/β-连环蛋白和BMP/Smad通路之间可能存在相互作用。

项目成果

Tumor Suppressor Genes: Functions, Regulation and Health Effects

肿瘤抑制基因：功能、调节和健康影响

• 发表时间: 2013
• 作者: Tazawa H;Kagawa S;Fujiwara T
• 通讯作者: Fujiwara T

DKK3 (dickkopf 3 homolog (Xenopus laevis))

• DOI: 10.4267/2042/51533
• 发表时间: 2013-09
• 期刊: Atlas of genetics and cytogenetics in oncology and haematology
• 作者: N. Katase;T. Nohno

Importance of Wnt8 lipid modification during formation of the Spemann's organizer in early Xenopus embryos

Wnt8 脂质修饰在早期非洲爪蟾胚胎 Spemann 组织者形成过程中的重要性

• 发表时间: 2012
• 作者: Masao Sakai;Eriko Motomura;Tomohiro Narita;Shin-ichiro Nishimatsu;Tsutomu Nohno;Hideaki Imai;Harukazu Nakamura& Shinichi Ohnuma;Tomohiro Narita
• 通讯作者: Tomohiro Narita

Wnt signaling during transition from cell proliferation to differentiation in myoblast cells

成肌细胞从细胞增殖到分化过程中的 Wnt 信号传导

• 发表时间: 2011
• 作者: Asai H;Morita S;Miyata S;Tanaka S,Nishimatsu S,Yamamura M,Nohno T
• 通讯作者: Tanaka S,Nishimatsu S,Yamamura M,Nohno T

Wnt signaling during transition from cell proliferation to differentiation in C2C12 cells.

C2C12 细胞从细胞增殖到分化过程中的 Wnt 信号传导。

• 发表时间: 2011
• 作者: Tanaka S;Terada K;Nohno T
• 通讯作者: Nohno T