Morphological and molecular cell biological study on cyto- and membrane-skeleton in skeletal muscle

用透射电子显微镜研究了肌原纤维与肌膜之间的锚定系统，用不同的方法清洗了细胞质中的可溶性蛋白。肌动蛋白和中间丝连接着肌原纤维和肌膜，其中许多连接到肌膜下的密度。这些丝状结构在MDX小鼠的骨骼肌中较少见。我们还研究了同步素的功能，它是骨骼肌中间丝的一种成分。通过实验，可能与骨骼肌的耐力和纤维类型的变化有关。此外，我们还研究了抗肌营养不良蛋白与同步素的关系。我们发现营养不良蛋白和同步素缺乏会影响生育能力。在斑马鱼胚胎发育过程中，受精后56~72小时在心脏中表达。

Using transmission electron microscope, we investigated anchoring system between myofibrils and sarcolemma by washing out cytoplasmic soluble proteins with various methods. Actin and intermediate filaments links between myofibrils and sarcolemma and many of them are anchored to the subsarcolemmal densities. These filamentous structures were less common in skeletal muscles of mdx mice. We also investigated the functions of synemin that is a component of intermediate filaments in skeletal muscle. Through experiments, it may relate to the endurance of skeletal muscle and the change of fiber types. Furthermore we investigated relationship of dystrophin and synemin. We found that deficiency of dystrophin and synemin affected fertility. During the development of Zebrafish embryo, it expressed in the heart from 56 to 72 hours after fertilization.