Molecular and Cell Biological Studies on Functional Proteins of Skeletal Muscle

骨骼肌功能蛋白的研究主要从两个方面进行。一个是膜蛋白的周转，我们集中在膜融合的控制蛋白，特别是VAMP（囊泡相关膜蛋白）家族蛋白。在大鼠成熟骨骼肌中，卫星细胞中发现VAMP2的强表达。注射心脏毒素的再生肌肉在注射后3天显示出明亮的VAMP2荧光。在胚胎发育过程中，于胚胎第12天（E12）出现在体节的成肌细胞中，并于胚胎第16天在多核肌管中保持阳性。VAMP2可能参与肌生成。在成熟的骨骼肌中，VAMP1位于神经肌肉接头的神经末梢，VAMP3位于肌内膜和肌周的内皮细胞。另一个是骨骼肌细胞骨架蛋白的体内功能分析。尝试产生基因敲除小鼠。已经获得杂合子小鼠，现在正在交配以获得纯合子动物。

Functional proteins of skeletal muscles were studied firm two aspects. One is the turnover of membrane proteins and we concentrated on the control proteins of membrane fusion, especially VAMP (Vesicle Associate Membrane Proteins) family proteins. In mature skeletal muscle of the rat, strong expression of VAMP2 was found in satellite cells. Regenerating muscles which had been injected cardiotoxin showed bright VAMP2 fluorescence at 3 days after injection. During ontogenesis, it appeared in myoblasts of the somites at embyonic day 12 (E12), and remained positive in multi-nucleated myotube at E16. VAMP2 may be involved in myogenesis. In mature skeletal muscles, VAMP1 was found at the nerve terminals of neuromuscular junctions and VAMP3 in endothelial cells of endo- and peri-mysium. The other is an analysis of I vivo function of a cytoskeletal protein of the skeletal muscle. Knockout mice were tried to be generated Heterozygote mice have been obtained and are now under mating to get homozygote animals.