Molecular cell biological studies on the functional molecules in skeletal muscle.

骨骼肌纤维收缩时，肌膜受到拉伸强度的影响，有时会造成肌膜撕裂。修复是通过囊泡融合进行的。但用于修复的囊泡的起源尚不清楚。我们研究了骨骼肌中的VAMPs（囊泡相关膜蛋白），发现VAMP2是肌肉分化的独特标记，而VAMP5在成熟肌纤维中起作用。此外，我们还研究了β -突触蛋白在体内骨骼肌中的功能，它不仅是中间细丝的组成成分，而且是细丝与肌膜之间的连接分子。我们发现，在通常的饲养条件和适度的运动负荷下，β -突触素对肌肉的发育和维持不是必需的。

Sarcolemma is exposed to tensile strength when skeletal muscle fiber contracts and it sometimes makes tears in sarcolemma. The repair is carried out by the fusion of vesicles. But the origin of the vesicles for repair is not known. We studied VAMPs (vesicle associated membrane proteins) in skeletal muscles and found that VAMP2 is a unique marker for muscle differentiation and VAMP5 works in mature muscle fibers. In addition, we studied the functions of beta-synemin in skeletal muscles in vivo which is not only a constituent of intermediate filaments but also a link molecule between the filaments and sarcolemma. We found that beta-synemin is not necessary for muscle development and maintenance under usual breeding condition as well as moderate exercise loading.