Electron microscopic analysis of lipid clusters and membrane proteins in plasma membrane.

质膜中脂质簇和膜蛋白的电子显微镜分析。

• 批准号: 23590251
• 负责人: MURATE Motohide
• 金额: $ 3.41万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590251/
• 关键词: 脂質ドメイン; フリーズフラクチャー; 脂質結合タンパク質; 血小板; マイクロパーティクル; スフィンゴミエリン; ホスファチジルセリン; 細胞膜; 電子顕微鏡; 脂質ラフト; 透過電子顕微鏡; リポソーム; ホスファチジルイノシトール-4,5-2リン酸; 免疫電子顕微鏡法; フリーズフラクチャー法; カベオラ; リン脂質; コレステロール

We examined transbilayer and lateral lipid distribution in plasma membrane using recently developed peptide/proteins that selectively bind specific lipids or lipid clusters. The liposome experiments demonstrated that the lipids were effectively detected by the probes even when the membrane concentration was as low as 1 mol%, and the probes bound similarly to the model membranes that mimic outer or inner leaflet lipid environment.Next, we examined the distribution of sphingomyelin and phosphatidylserine in resting and thrombin-stimulated platelets as well as platelet microparticles that are highly procoagulant. Our results indicate that the scrambled membranes are selectively released in the form of microparticles in thrombin-activated platelets and suggest the idea that the exposure of phosphatidylserine in inner leaflet to the outer triggers the vesiculation of platelets.

我们研究transbilayer和横向脂质分布在质膜使用最近开发的肽/蛋白质，选择性地结合特定的脂质或脂质簇。脂质体的实验表明，即使当膜浓度低至1摩尔%的探针，脂质被有效地检测到，和探针结合类似的模型膜，模仿外部或内部小叶脂质environment.Next，我们研究了分布的鞘磷脂和磷脂酰丝氨酸在静息和凝血酶刺激的血小板以及血小板微粒，是高度促凝。我们的研究结果表明，混乱的膜被选择性地释放在凝血酶激活的血小板中的微粒的形式，并建议的想法，即暴露的磷脂酰丝氨酸在内部小叶的外部触发血小板的囊泡化。

项目成果

小林脂質生物学研究室

小林脂质生物实验室

Transformation of phosphatidylethanolamine- and phosphatidylserine-containing membranes by phospholipase C beta 1

磷脂酶 C beta 1 转化含磷脂酰乙醇胺和磷脂酰丝氨酸的膜

• 发表时间: 2013
• 作者: Tajima T;Inaba T;Kishimoto T;Abe M;Murate M;Makino A;Sakai S;Hullin-Matsuda F;Ishitsuka R;Ikeda Y;Takeoka S;Kobayashi T
• 通讯作者: Kobayashi T

Molecular mechanism of the inhibition of cardiolipin polymorphism by 10-N-Nonyl Acridine Orange

10-N-壬基吖啶橙抑制心磷脂多态性的分子机制

• 发表时间: 2012
• 作者: Greimel P;Murate M;Hullin-Matsuda F;Nabetani T;Iwamoto K;Ito K;Takata M;Kobayashi M;Takahashi H;Kobayashi T
• 通讯作者: Kobayashi T

Revisiting plasma membrane lipid asymmetry.

重新审视质膜脂质不对称性。

• 发表时间: 2012
• 作者: Motohide Murate;Mitsuhiro Abe;Masato Umeda and Toshihide Kobayashi.
• 通讯作者: Masato Umeda and Toshihide Kobayashi.

Visualization of lipids and lipid domains

脂质和脂质域的可视化

• 发表时间: 2011
• 作者: 小林俊秀;阿部充宏;岸本拓磨;牧野麻美;Hullin-Matsuda F;石塚玲子;村手源英
• 通讯作者: 村手源英