Paracellular permeability of tight junction examined by different combination of claudins or claudin-1 mutants.

我们通过对小鼠直肠CMT 93-I和CMT 93-II细胞的比较分析发现，claudin-2的表达引起细胞旁通透性的增加。这表明紧密连接功能可能取决于细胞中表达的紧密连接蛋白种类的组合。我们发现，两个半胱氨酸残基（54 C，64 C）保守在第一个细胞外环的claudin-1是必要的形成紧密连接链和54 C丙氨酸取代引起跨上皮电阻增加。

We found by comparative analysis of mouse rectum CMT93-I and -II cells that expression of claudin-2 caused increase of paracellular permeability. This suggests that tight junction function may depend on combination of claudin species expressed in the cells. We found that two cysteine residues (54C, 64C) conserved in the first extracellular loop of claudin-1 were necessary for the formation of tight junction strands and that replacement of 54C to alanine caused increase of transepithelial electrical resistance.