Basic research on persistent infection of HCV and molecular mechanisms of RNA interference

TRAX是RNA干扰(RNAi)过程中RNA诱导沉默复合体(RISC)的一个组成部分。鉴于Trax在RNAi机制中的重要作用，我们分析了其在丙型肝炎病毒持续感染机制中的作用。在本研究期间，虽然我们不能证明Trax参与了丙型肝炎病毒的感染，但对于Trax分子的性质，我们得到了以下结果：1)Trax蛋白本身不能在细胞内表达。2)Trax蛋白通常以与Translin蛋白的复合体形式存在，以逃避泛素依赖的蛋白酶体降解途径。3)Trax蛋白的C-末端容易被降解。

TRAX (Translin-associated factor X) is known as a component of RISC (RNA-induced silencing complex) that is involved in RNA interference (RNAi). Given the significant role of TRAX on RNAi machinery, we analyzed its effect on the mechanism of persistent infection of hepatitis C virus (HCV). Although we could not prove the involvement of TRAX in HCV infection during the study period, we obtained the following results for the nature of TRAX molecule: 1)TRAX protein alone cannot be cellularly expressed. 2)TRAX protein usually exists as a complex with Translin protein to escape from ubiquitin-independent proteasome degradation pathway 3) The C-terminal region of TRAX protein is susceptible to degradation.