The involvement of sphingolipid metabolism in anti cancer drug sensitivity of malignant cells
鞘脂代谢参与恶性细胞抗癌药物敏感性
基本信息
- 批准号:23590667
- 负责人:
- 金额:$ 3.49万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2011
- 资助国家:日本
- 起止时间:2011 至 2013
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In the first year, we analyzed the mechanism of GAP43 expression during GDNF treatment of a neuronal cel line, TGW. Our analysis revealed that GDNF induced SPHK1 expression through RET receptor, followed by S1P secretion and S1P1/3 receptor activation. We further found that GAP43 expression was due to the increased CREB transcription factor binding to the GAP43 5'-promoter region.Next, we analyzed the regulatory mechanism of neutral sphingomyelinase 2 and neutral ceramidase with all trans retinoid acid (ATRA). Our analysis revealed that activated Sp1 transcription factor and decreased GATA2 were responsive for the observed changes in treated MCF-7 and SH-SY5Y cells, respectively.In the third year, we analyzed the mechanism of high SPHK1 expression of mouse Friend cells. We found that c-MYB is responsible for this overepression and that chemical inducer, HMBA, rapidly decreased SPHK1 expression, which is at least partially responsible for this terminal differentiation process.
在第一年,我们分析了GDNF处理神经细胞系TGW时GAP43表达的机制。我们的分析显示GDNF通过RET受体诱导SPHK1表达,随后S1P分泌和S1P1/3受体激活。我们进一步发现,GAP43的表达是由于CREB转录因子与GAP43 5'-启动子区域结合增加所致。接下来,我们分析了全反式维甲酸(ATRA)对中性鞘磷脂酶2和中性神经酰胺酶的调控机制。我们的分析显示,Sp1转录因子的激活和GATA2的减少分别对MCF-7和SH-SY5Y细胞的变化有响应。第三年,我们分析了小鼠Friend细胞中SPHK1高表达的机制。我们发现c-MYB负责这种过表达,而化学诱导剂HMBA迅速降低SPHK1的表达,这至少部分负责这种终端分化过程。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sphingosine kinase 1 expression is downregulated during differentiation of Friend cells due to decreased c-MYB.
由于 c-MYB 减少,鞘氨醇激酶 1 表达在 Friend 细胞分化过程中下调。
- DOI:10.1016/j.bbamcr.2013.01.001
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:Mizutani N;Kobayashi M;Sobue S;Ichihara M;Ito H;Tanaka K;Iwaki S,Fujii S,Ito Y;Tamiya-Koizumi K;Takagi A;Kojima T;Naoe T;Suzuki M;Nakamura M;Banno Y;Nozawa Y;Murate T.
- 通讯作者:Murate T.
アンドロジェン反応性ヒト前立腺がん細胞株LNCaPにおける酸性セラミダーゼ発現調節機序
雄激素反应性人前列腺癌细胞系LNCaP中酸性神经酰胺酶表达的调控机制
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:東田修二;大野彩;高橋祐介;奥橋佑基;伊藤真以;Toru Takano;登勉;井上みなみら
- 通讯作者:井上みなみら
Hybrid liposomes affect cellular lipids
混合脂质体影响细胞脂质
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:2.7
- 作者:Hiroyuki Yasui;Yutaro Natsume;and Yutaka Yoshikawa;Cao K. et al.
- 通讯作者:Cao K. et al.
C-Myb is the major regulator of sphingosine kinase 1 expression of Friend cells
C-Myb 是 Friend 细胞鞘氨醇激酶 1 表达的主要调节因子
- DOI:
- 发表时间:2012
- 期刊:
- 影响因子:0
- 作者:Ohyama Yoshiaki;Kurabayashi Masahiko;Mizutani N. et al.
- 通讯作者:Mizutani N. et al.
Sphk 1 of Friend cells was decreased by HMBA
HMBA 降低 Friend 细胞的 Sphk 1
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:Yamada;H.;Takano;T.;Kihara;M.;Hirokaw,a M.;Yoshida;H.;Watanabe;M.;Iwatani;Y.;Hidaka;Y.;.Miyauchi;A;小林 美沙ら
- 通讯作者:小林 美沙ら
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MURATE Takashi其他文献
A clinical laboratory test detecting antithrombin resistance of the new thrombophilia
检测新型血栓形成倾向的抗凝血酶抵抗的临床实验室测试
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
MURATA Moea;TAKAGI Akira;SUZUKI Atsuo,TAKAGI Yuki;KATO Io;ANDO Yumi;MURATE Takashi;MATSUSHITA Tadashi;SAITO Hidehiko;KOJIMA Tetsuhito - 通讯作者:
KOJIMA Tetsuhito
MURATE Takashi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MURATE Takashi', 18)}}的其他基金
The alteration of the sphingolipid metabolism of anti-cancer drug resistant tumor cells and the overcome of these resistance by the phytochemicals
抗癌药物耐药肿瘤细胞鞘脂代谢的改变以及植物化学物质克服这些耐药性
- 批准号:
17K09025 - 财政年份:2017
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Stress response of cancer cells focusing the sphingolipid metabolism and its modulation by food ingredients
癌细胞的应激反应聚焦于鞘脂代谢及其受食物成分的调节
- 批准号:
26460674 - 财政年份:2014
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Involvement and abnormality of lysosphingolipid metabolic enzymes in malignant diseases
溶血鞘脂代谢酶在恶性疾病中的参与及异常
- 批准号:
20590566 - 财政年份:2008
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Aberrant expression of sphingosine kinase and its pathological significance of malignant tumors and neuronal degenerative diseases
鞘氨醇激酶的异常表达及其在恶性肿瘤和神经退行性疾病中的病理意义
- 批准号:
18590526 - 财政年份:2006
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analysis of the efficiency of the separation of normal hematopoietic stem cells of myelodysplastic syndrome for auto stem cell transplantation
骨髓增生异常综合征正常造血干细胞分离用于自体干细胞移植的效率分析
- 批准号:
11670991 - 财政年份:1999
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
THE ANALYSIS OF DIFFERENTIATION INDUCTION OF CYTOKINE DEPENDENT LEUKEMIA CELLL LINE AND THE IDENTIFICATION OF MYELOID LINEAGE APESIFIC TRANSCRIPTION FACTORS
细胞因子依赖性白血病细胞系分化诱导分析及髓系非转录因子鉴定
- 批准号:
07671192 - 财政年份:1995
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Structure and function of metabolic enzyme assemblies
代谢酶组装体的结构和功能
- 批准号:
10621612 - 财政年份:2023
- 资助金额:
$ 3.49万 - 项目类别:
Structural Analysis of a Metabolic Enzyme Complex: Insights into Coupling between Glycolysis and Fermentation
代谢酶复合物的结构分析:深入了解糖酵解与发酵之间的耦合
- 批准号:
23K05667 - 财政年份:2023
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The role of cholesterol metabolic enzyme CYP46A1 and its metabolite 24S-hydroxycholesterol in ischemic stroke.
胆固醇代谢酶CYP46A1及其代谢物24S-羟基胆固醇在缺血性脑卒中中的作用。
- 批准号:
10503336 - 财政年份:2022
- 资助金额:
$ 3.49万 - 项目类别:
The role of cholesterol metabolic enzyme CYP46A1 and its metabolite 24S-hydroxycholesterol in ischemic stroke.
胆固醇代谢酶CYP46A1及其代谢物24S-羟基胆固醇在缺血性脑卒中中的作用。
- 批准号:
10629388 - 财政年份:2022
- 资助金额:
$ 3.49万 - 项目类别:
Understanding the structure and function of an important human metabolic enzyme.
了解重要的人类代谢酶的结构和功能。
- 批准号:
2589476 - 财政年份:2021
- 资助金额:
$ 3.49万 - 项目类别:
Studentship
Stratification of patients with MDS for epigenetic agent from the viewpoint of cholesterol metabolic enzyme
从胆固醇代谢酶的角度对MDS患者表观遗传因素进行分层
- 批准号:
21K07404 - 财政年份:2021
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
New approaches to combat CNS inflammation in Veterans: Targeting a metabolic enzyme in demyelinating disease
对抗退伍军人中枢神经系统炎症的新方法:针对脱髓鞘疾病中的代谢酶
- 批准号:
10427137 - 财政年份:2019
- 资助金额:
$ 3.49万 - 项目类别:
New approaches to combat CNS inflammation in Veterans: Targeting a metabolic enzyme in demyelinating disease
对抗退伍军人中枢神经系统炎症的新方法:针对脱髓鞘疾病中的代谢酶
- 批准号:
9565043 - 财政年份:2019
- 资助金额:
$ 3.49万 - 项目类别:
New approaches to combat CNS inflammation in Veterans: Targeting a metabolic enzyme in demyelinating disease
对抗退伍军人中枢神经系统炎症的新方法:针对脱髓鞘疾病中的代谢酶
- 批准号:
10084232 - 财政年份:2019
- 资助金额:
$ 3.49万 - 项目类别:
Introducing inter-individual differences into the biological monitoring of organophosphorus pesticide: Elucidating the effects of metabolic enzyme activity to the amount of urinary metabolite.
将个体差异引入有机磷农药生物监测:阐明代谢酶活性对尿代谢物量的影响。
- 批准号:
19K19414 - 财政年份:2019
- 资助金额:
$ 3.49万 - 项目类别:
Grant-in-Aid for Early-Career Scientists














{{item.name}}会员




