The standardization of forensic genetic analysis of degraded DNA samples

点击翻译按钮获取中文摘要

The problem with which forensic scientists have recently been confronted involves the technical difficulty of analyzing degraded DNA samples. We designed minimultiplex PCR systems using single-base extension reactions to identify Japanese Y chromosome haplogroups and mitochondrial DNA haplogroups. We selected a group of 22 Y chromosome single nucleotide polymorphisms(SNPs) and 46 mitochondrial DNA SNPs, respectively. We designed primers to render amplicons of <150 bp. Applying these systems, we classified the Japanese population into haplogroups and confirmed the applicability of these system in forensic degraded DNA analysis.