A basic study for evaluation method of brain function on forensic autopsy cases.

法医尸检案件脑功能评价方法的基础研究

• 批准号: 23590851
• 负责人: NISHIMURA Akiyoshi
• 金额: $ 3.33万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590851/
• 关键词: 法医病理学; dementia; schizophrenia; hippocampus; lectin; erythropoietin receptor; NOS; N-Cadherin; chondroitin sulfate; Alzheimer; Braak stage; dopaminergic; serotonin; cholinergic; microglia; astrocyte; レクチン; アルツハイマー; Gallias-Braak; 海馬歯状回

Autopsied brains with dementia were examined by means of immunohistochemical staining method using 17 antibodies, related with neuronal degeneration, cellular damage and oxidative stress. Especially, a large number of SPDs were stained by antibodies of YKL-39, Tau, CS and HSP70. Furthermore, development frequency of SPDs between by mean of NOS and GFAP, NOS and CS, ApoE and CS, Tau and HSP70, and CS and HSP70, showed positive correlations. Substances relating with dementia are detected in same SPD by mean of multi fluorescent immunehistochemical staining visualized by confocal laser microscopy. Although, SPD were related with apoptosis of neurons in schizophrenia, SPD might come from not only neuronal degeneration but also glial in degenerative dementia. SPDs with reactivity of EAAC1 and/or alpha-syn related with neuronal degeneration were detected in the early stages and they with reactivity of S-100 and/or YKL-40 related with astrocytes were detected in the middle or later stages.

采用免疫组化染色法检测痴呆脑组织中与神经元变性、细胞损伤和氧化应激相关的17种抗体。特别是，大量spd被YKL-39、Tau、CS和HSP70抗体染色。NOS与GFAP、NOS与CS、ApoE与CS、Tau与HSP70、CS与HSP70的平均spd发生频率呈正相关。用激光共聚焦显微镜多荧光免疫组织化学染色检测同一SPD中与痴呆相关的物质。虽然SPD与精神分裂症的神经元凋亡有关，但SPD可能不仅来自神经元变性，还可能来自退行性痴呆的神经胶质。早期检测到EAAC1和/或α -syn反应性与神经元变性相关的SPDs，中晚期检测到S-100和/或YKL-40反应性与星形胶质细胞相关的SPDs。

项目成果

ヒト剖検脳の海馬歯状回分子層における球状沈着物の免疫組織化学的検討

人体尸检脑海马齿状回分子层球形沉积物的免疫组织化学研究

• 发表时间: 2013
• 作者: 山田茉樹;石上安希子;徳永逸夫;西村明儒
• 通讯作者: 西村明儒

Histopathological investigations of lectin positive spherical deposits detected in the molecular layer of the hippocampal formation with neurofibrillary tangles

在海马结构分子层检测到的凝集素阳性球形沉积物与神经原纤维缠结的组织病理学研究

• 发表时间: 2014
• 作者: Akiyoshi NISHIMURA;Akiko ISHIGAMI,Hiromitsu;KURATA;Itsuo TOKUNAGA
• 通讯作者: Itsuo TOKUNAGA

Histopathological investigations of lectin positive spherical deposits detected in the molecular layer of the hippocampal formation with neurofibrillary tangles.

在海马结构分子层中检测到的凝集素阳性球形沉积物与神经原纤维缠结的组织病理学研究。

• 发表时间: 2014
• 作者: Akiyoshi NISHIMURA;Akiko ISHIGAMI，Hiromitsu;KURATA;Itsuo TOKUNAGA
• 通讯作者: Itsuo TOKUNAGA