Development of the molecular imaging and the therapy of the unstable plaque targeting activated macrophages

针对活化巨噬细胞的不稳定斑块的分子影像和治疗的发展

• 批准号: 23591105
• 负责人: MIYATA MASAAKI
• 金额: $ 2.91万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591105/
• 关键词: 動脈硬化; 葉酸レセプターβ; マクロファージ; 活性化マクロファージ; 分子イメージング

The folic acid receptor beta (FR-beta) attracts attention as a marker of activated macrophages. We found that the activated macrophage which expressed FR-beta was present in the arteriosclerosis lesion of the arteriosclerosis model mouse. We administrated the recombinant immunotoxin which coupled Pseudomonas aeruginosa toxin to anti-FR-beta monoclonal antibody into the arteriosclerosis model mice, selectively killed the activated macrophages, and demonstrated that it reduced arteriosclerotic lesion.In addition, we established a sandwich ELISA assay using anti-FR-beta monoclonal antibody and measured the soluble FR-beta concentration of human serum. The patients with aortic aneurysms showed a higher serum FR-beta level compared with the normal control. The measurement of the soluble FR-beta concentration is suggested to be useful for the diagnosis of atherosclerosis.

叶酸受体β (fr - β)作为活化巨噬细胞的标记物引起了人们的关注。我们发现在动脉硬化模型小鼠的动脉硬化病变中存在表达fr - β的活化巨噬细胞。我们将铜绿假单胞菌毒素与抗fr - β单克隆抗体偶联的重组免疫毒素注入动脉硬化模型小鼠，选择性地杀死活化的巨噬细胞，并证明其能减轻动脉硬化病变。此外，我们建立了抗fr - β单克隆抗体夹心ELISA法，测定了人血清中可溶性fr - β的浓度。动脉瘤患者血清fr - β水平高于正常对照组。可溶性fr - β浓度的测定被认为对动脉粥样硬化的诊断有用。

项目成果

Topic 10「動脈硬化治療の最前線」Novel therapy for atherosclerosis targeting folate receptorβ-expressing activated macrophages

主题10“动脉硬化治疗的最前沿”以表达叶酸受体β的活化巨噬细胞为靶点的动脉粥样硬化新疗法

• 发表时间: 2012
• 作者: Miyata M;Furusho Y;Tei C
• 通讯作者: Tei C

Novel therapy for atherosclerosis targeting folate receptor β-expressing activated macrophages

针对表达叶酸受体β的激活巨噬细胞的动脉粥样硬化新疗法

• 发表时间: 2012
• 作者: Miyata M;Furusho Y;Tei C
• 通讯作者: Tei C

Targeting FRβ-expressing macrophages reduced atherosclerosis in apolipoprotein-E-deficient mice

靶向表达 FRβ 的巨噬细胞可减少载脂蛋白 E 缺陷小鼠的动脉粥样硬化

• 发表时间: 2012
• 作者: Furusho Y;Miyata M;Akasaki Y;Hamada N;Miyauchi T;Ikeda Y;Hamasaki S;Tei C
• 通讯作者: Tei C

Therapeutic agent for arteriosclerosis or arteriosclerotic diseae, and diagnositic agent for arteriosclerosis or arteriosclerosic disease

动脉硬化或动脉硬化疾病的治疗剂以及动脉硬化或动脉硬化疾病的诊断剂

• 发表时间: 2013

Influence of gefitinib and erlotinib on apoptosis and c-MYC expression in H23 lung cancer cells.

吉非替尼和厄洛替尼对H23肺癌细胞凋亡和c-MYC表达的影响。

• 发表时间: 2013
• 期刊: Anticancer Res
• 影响因子: 2
• 作者: Suenaga M;Yamamoto M;Tabata S;Itakura S;Miyata M;Hamasaki S;Furukawa T
• 通讯作者: Furukawa T