MOLECULAR PHYSIOLOGY OF SUCCINIC ACID ACID BASED FERMENTATIONS IN ANAEROBES
MOLECULAR PHYSIOLOGY OF SUCCINIC ACID ACID BASED FERMENTATIONS IN ANAEROBES
批准号:
6258879
负责人:
J GREGORY ZEIKUS
金额:
$0.03万
依托单位国家:
美国
项目类别:
财政年份:
1997
资助国家:
美国
项目状态:
已结题
起止时间:
1997-06-01 至 1999-11-30
中文摘要
琥珀酸,第一大宗商品化学品,其中
英文摘要
Succinic acid, the first large quantity commodity chemical, which
can be made by fermentation of plant biomass substrates, is presently
produced from petrochemical feedstocks. Succinic acid is an
intermediate of the citric acid cycle, and many anaerobic bacteria
form succinate as a fermentation product. The bacterium
Anaerobiospirillum succiniciproducens is able to produce 35-50 g/l of
succinate, but Actinobacillus sp. produces 130Z 65-80g/l.
Phosphoenolpyruvate (PEP) carboxykinase is the key, regulated enzyme
condensing PEP and CO2 to form oxaloacetate, in the pathway leading to
succinate. We have successfully cloned and expressed A.
succiniciproducens PEP carboxykinase in E. coli. This 60.9 kDa
monomeric recombinant enzyme, containing an N-terminal His tag, was
purified and used for the development of an MS-based protein
sequencing method. At present we have purified the Actinobacillus sp
130Z PEP carboxykinase. TMPP-Ac charge derivatization and peptide
sequencing by MALDI-PSD-MS is being used to assist in cloning the
corresponding gene.
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会议论文
AGRICULTURE RAW MATERIALS USE: CLONING EXPRESSION & CHARACTERIZATION
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批准号:6248452
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项目类别:
-
资助金额:$0.46万
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财政年份:1997
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负责人:J GREGORY ZEIKUS
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依托单位:
海外基金