Development of novel diagnostic and therapeutic biomarkers for hypereosinophilic syndrome

高嗜酸性粒细胞综合征（HES）是一种异质性疾病，其特征是长期嗜酸性粒细胞增多，原因不明。我们明确了参与本研究的患者的临床鉴别诊断，并通过RT-PCR检测FIP1L1-PDGFRA嵌合mRNA的存在。此外，我们还通过质谱法（nanoLC-MS/MS）对从不同患者外周血中提取的磷酸肽进行了磷蛋白组学研究，发现了与嗜酸性粒细胞增多症相关的不同谱。这些信息可用于HES的鉴别诊断。

Hypereosinophilic syndrome (HES) is a heterogeneous disorders characterized by prolonged eosinophilia with causes unknown. We clarified the clinical differential diagnosis of the patients who participated in this study, and tested for the existence of FIP1L1-PDGFRA chimeric mRNA by RT-PCR. Moreover, we also performed phosphoproteomic studies by mass spectrometry (nanoLC-MS/MS) using the phsphopeptides extracted from peripheral blood of various patients and found the different profiles with which correlate the underlying condition of eosinophilia. This information of phosphopeptides could be useful in the differential diagnosis of HES.