Role of recombinant nicked beta2-glycoprotein I domain V in angiogenesis

重组切口β2-糖蛋白I结构域V在血管生成中的作用

• 批准号: 23591431
• 负责人: YASUDA Shinsuke
• 金额: $ 3.24万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591431/
• 关键词: 血管新生; アンギオスタチン; β2-グリコプロテインI; 抗リン脂質抗体症候群; 血栓症

Previously we reported nicked beta2-glycoprotein I (b2GPI) binds to angiostatin via its domain V and exerts pro-angiogenic effect (Nakagawa et al. Blood 2009). The aim of this study was to investigate the role of b2GPI domain V in angiogenesis. Recombinant b2GPI domain V was generated in mammalian cell expression system and bacteria. Purification of the product was done using anti-FLAG column or nickel column. Binding between intact/nicked b2GPI domain V and angiostatin 4.5 (AS4.5) was evaluated using surface plasmon resonance. Nicked b2GPI domain V specifically bound onto AS4.5 at KD of ~ 5 x 10-8M, whereas intact b2GPI domain V showed no specific binding. Cell proliferation assay using human umbilical endothelial cells (HUVECs) were performed in the presence or absence of intact/nicked b2GPI domain V. Both intact and nicked b2GPI domain V slightly inhibited cell proliferation at 0.4uM.In conclusion, nicked b2GPI domain V bound to AS4.5, as observed in nicked form of whole b2GPI.

先前我们报道了缺口β -糖蛋白I （b2GPI）通过其结构域V与血管抑制素结合并发挥促血管生成作用（Nakagawa等）。血2009)。本研究旨在探讨b2GPI结构域V在血管生成中的作用。重组b2GPI结构域V在哺乳动物细胞表达系统和细菌中生成。采用反flag柱或镍柱对产物进行纯化。利用表面等离子体共振评估完整/缺口b2GPI结构域V与血管抑制素4.5 （AS4.5）之间的结合。缺失的b2GPI结构域V在KD为~ 5 × 10-8M时特异性结合到AS4.5上，而完整的b2GPI结构域V没有特异性结合。使用人脐带内皮细胞（HUVECs）进行细胞增殖实验，在存在或不存在完整的/缺口的b2GPI结构域V的情况下，完整的和缺口的b2GPI结构域V在0.4uM时均能轻微抑制细胞增殖。综上所述，b2GPI的切口结构域V与AS4.5结合，在整个b2GPI中观察到切口形式。

项目成果

The involvement of CD36 in monocyte activation by antiphospholipid antibodies.

CD36 参与抗磷脂抗体激活单核细胞。

• DOI: 10.1177/0961203313490242
• 发表时间: 2013
• 期刊: Lupus
• 影响因子: 2.6
• 作者: Kato M;Atsumi T;Oku K;Amengual O;Nakagawa H;Fujieda Y;Otomo K;Horita T;Yasuda S;Koike T.
• 通讯作者: Koike T.

Role of β2-glycoprotein I Domain V in Angiogenesis

β2-糖蛋白 I 结构域 V 在血管生成中的作用

• 发表时间: 2013
• 作者: Yasuda S;Kubota A;Nakagawa H;Kuroki K;Fujieda Y;Bohgaki T;Amengual O;Horita T;Maenaka K;Atsumi T
• 通讯作者: Atsumi T

Diagnosis and treatment of RA in Japan

日本RA的诊断和治疗

• 发表时间: 2013
• 作者: Matsumoto;M.;Nishikawa;Y.;Mouri;Y.;Hirota;F.;Nishijima;H;Yasuda S
• 通讯作者: Yasuda S

Disseminated intravascular coagulation in the presence of antiphospholipid antibodies : differential diagnosis from catastrophic or microangiopathic antiphospholipid syndrome

抗磷脂抗体存在下的弥散性血管内凝血：与灾难性或微血管病性抗磷脂综合征的鉴别诊断

• 发表时间: 2013
• 期刊: Intern Med
• 作者: 細野治;上原昌晃;松宮遼;吉川賢忠;小林浩;鈴木幸男;田中廣壽;Yasuda S
• 通讯作者: Yasuda S

Essential role of the p38 mitogen-activated protein kinase pathway in tissue factor gene expression mediated by the phosphatidylserine-dependent antiprothrombin antibody.

p38 丝裂原激活蛋白激酶途径在磷脂酰丝氨酸依赖性抗凝血酶原抗体介导的组织因子基因表达中的重要作用。

• DOI: 10.1093/rheumatology/ket234
• 发表时间: 2013
• 期刊: Rheumatology (Oxford).
• 作者: Oku K;Amengual O;Zigon P;Horita T;Yasuda S;Atsumi T.
• 通讯作者: Atsumi T.