The role of the adipocyte function after the surgical stress: the effectiveness of the pioglitazone administration

手术应激后脂肪细胞功能的作用：吡格列酮给药的有效性

• 批准号: 23591880
• 负责人: MATSUTANI Takeshi
• 金额: $ 3.24万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591880/
• 关键词: 脂肪細胞; 手術侵襲; アディポサイトカイン; ピオグリタゾン

To investigate the effect of PPAR-gamma (PPARg) agonist on cecal ligation and puncture (CLP)-induced adipose-tissue inflammation and lung injury in mice. Mice were assigned to 3 groups: 1) CLP group, 2) PPARg agonist-treated CLP group, or 3) sham group. PPARg agonist was injected intraperitonally for 7 days. TNF-alpha (TNF) levels in serum and peritoneal lavage fluid were significantly higher in the CLP group than in the PPARg agonist-treated CLP group. PPARg agonist treatment decreased the TNF mRNA expression levels as compared with the CLP group. Histopathological analysis of lung tissue revealed significantly increased numbers of TUNEL-positive cells in the CLP group as compared with the sham-operated control group. PPARg agonist treatment reduced the CLP-induced cell and tissue injury as compared with the CLP group. PPARg agonist effectively suggested to prevent lung injury caused by CLP-induced sepsis by maintaining the anti-inflammatory status of adipose tissue.

目的探讨PPARg激动剂对盲肠结扎穿孔（CLP）所致小鼠脂肪组织炎症和肺损伤的影响。将小鼠分为3组：1）CLP组，2）PPARg激动剂处理的CLP组，或3）假手术组。PPARg激动剂腹腔注射7天。CLP组血清和腹腔灌洗液中TNF-α（TNF）水平显著高于PPARg激动剂治疗的CLP组。PPARg激动剂治疗降低TNF mRNA表达水平与CLP组相比。肺组织的组织学分析显示CLP组中TUNEL阳性细胞的数量与假手术对照组相比显著增加。PPARg激动剂处理减少CLP诱导的细胞和组织损伤与CLP组相比。PPARg激动剂可通过维持脂肪组织的抗炎状态，有效预防CLP脓毒症所致的肺损伤。