Development of a new fluorescence imaging system for peritoneal and lymph node metastasis of GI tract cancers

开发用于胃肠道癌症腹膜和淋巴结转移的新型荧光成像系统

• 批准号: 23591952
• 负责人: ITO Yuichi
• 金额: $ 3.33万
• 依托单位: Aichi Cancer Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591952/
• 关键词: 蛍光イメージング; 腹腔鏡; ICG近赤外蛍光; 腹膜転移; リンパ節転移; 抗体プローブ; ICG蛍光; リンパ節高転移; 光イメージング; 分子イメージング; 胃がん・大腸がん

Peritoneal and lymph node metastasis is the most frequent pattern of recurrence after curative surgery for gastric cancer. However, such a recurrence is difficult to detect by conventional CT and MRI at an early stage. In the present study, we first developed a new lymph node metastasis model using GFP-tagged tumor cells. Second, we developed a new laparoscopic fluorescence device for detecting metastasis using ICG-labeled antibody as a probe. Using these mouse metastasis model and imaging device, we successfully detected peritoneal metastasis and lymph node metastasis at an early stage with high sensitivity and specificity.

腹膜和淋巴结转移是胃癌根治术后最常见的复发方式。然而，这种复发很难通过常规的CT和MRI在早期发现。在本研究中，我们首先利用GFP标记的肿瘤细胞建立了一种新的淋巴转移模型。其次，我们利用ICG标记的抗体作为探针，开发了一种新的用于检测转移的腹腔镜荧光装置。利用这些小鼠转移模型和成像设备，我们成功地对腹膜转移和淋巴结转移进行了早期检测，具有较高的敏感性和特异性。

项目成果

Establishment and characterization of novel gastric signet-ring cell and non signet-ring cell poorly differentiated adenocarcinoma cell lines with low and high malignant potential

• DOI: 10.1007/s10120-012-0149-2
• 发表时间: 2013-01-01
• 期刊: GASTRIC CANCER
• 影响因子: 7.4
• 作者: Murakami, Hiroki;Nakanishi, Hayao;Kodera, Yasuhiro
• 通讯作者: Kodera, Yasuhiro

Induction of a Pathological Complete Response by Four Courses of Neoadjuvant Chemotherapy for Gastric Cancer: Early Results of the Randomized Phase II COMPASS Trial

• DOI: 10.1245/s10434-013-3055-x
• 发表时间: 2014-01-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Yoshikawa, Takaki;Tanabe, Kazuaki;Sakamoto, Junichi
• 通讯作者: Sakamoto, Junichi