A clinical and basic research for developing human small hepatocyte bank using super-cooling program and generating mass hybrid mouse.

我们调查了应用临床肝再生治疗的基础研究和确保供体细胞获取的临床研究。在基础研究方面，我们建立了肝细胞移植肝再生治疗的大鼠模型。在这个模型中，我们揭示了移植细胞的细胞生理学，它产生了胆管衬里细胞。此外，我们还成功地提高了Nash肝硬变模型的存活率。在临床研究中，我们报告了供体细胞获取的好处和缺陷。我们还揭示了BTR、ATIII和GSA的临床价值。采用聚类分析对各种肝功能指标进行分类。此外，在技术报告中，我们开发了一种用于进行安全的右肝切除的外科设备(J am Col Surg：正在出版中)。

We investigated that basic research for applying clinical liver regenerative therapy and clinical research for securing donor cell procurement. In the basic research, we developed a rat model of liver regenerative therapy by hepatocyte transplantation. In this model, we revealed a cell physiology of the transplanted cells which generated bile duct linage cells. In addition, we succeded to increase survival rate in NASH liver cirrhotic model. In the clinical research, we reported a benefit and default of donor cell procurement. We also revealed that a clinical value of BTR, ATIII, and GSA. We classified various liver functional indicators using clustering analysis. Furthermore, in technical reports, we developed a surgical devise for conducting safety right liver hepatectomy (J Am Col Surg : in press).