A clinical and basic research for developing human small hepatocyte bank using super-cooling program and generating mass hybrid mouse.

利用过冷程序建立人小肝细胞库并产生大规模杂交小鼠的临床和基础研究。

• 批准号: 23591993
• 负责人: MIZUGUCHI TORU
• 金额: $ 3.16万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591993/
• 关键词: 肝臓外科学; 肝細胞移植; 再生置換; 肝機能; 肝幹細胞; 肝切除術; 自己組織化; 生体機能利用; 移植; 再生医療; 応用動物; アミノ酸; 腹腔鏡

We investigated that basic research for applying clinical liver regenerative therapy and clinical research for securing donor cell procurement. In the basic research, we developed a rat model of liver regenerative therapy by hepatocyte transplantation. In this model, we revealed a cell physiology of the transplanted cells which generated bile duct linage cells. In addition, we succeded to increase survival rate in NASH liver cirrhotic model. In the clinical research, we reported a benefit and default of donor cell procurement. We also revealed that a clinical value of BTR, ATIII, and GSA. We classified various liver functional indicators using clustering analysis. Furthermore, in technical reports, we developed a surgical devise for conducting safety right liver hepatectomy (J Am Col Surg : in press).

我们对肝再生治疗临床应用的基础研究和保证供体细胞获取的临床研究进行了探讨。在基础研究方面，我们建立了肝细胞移植治疗肝再生的大鼠模型。在这个模型中，我们揭示了移植细胞产生胆管系细胞的细胞生理学。此外，我们还成功提高了NASH肝硬化模型的生存率。在临床研究中，我们报道了供体细胞获取的利弊。我们还揭示了BTR、ATIII和GSA的临床价值。我们使用聚类分析对各种肝功能指标进行分类。此外，在技术报告中，我们开发了一种手术装置，用于进行安全的右肝切除术（J Am Col surgery: in press）。

项目成果

Prognostic impact of preoperative the branched-chain amino acid to the tyrosine ratio in hepatocellular carcinoma patients after initial hepatectomy

术前支链氨基酸与酪氨酸比值对肝细胞癌初次肝切除术后患者预后的影响

• DOI: 10.1007/s11605-011-1566-y
• 发表时间: 2011
• 期刊: Journal of Gastrointestinal Surgery
• 影响因子: 3.2
• 作者: Mizuguchi T;et al
• 通讯作者: et al

Hepatic Progenitor Cell Transplantation for Improving Survival after Liver Resection of Rat Non-alcoholic Steatocirrhotic Liver Model

肝祖细胞移植提高大鼠非酒精性脂肪肝硬化模型肝切除后的存活率

• 发表时间: 2012
• 作者: Nakamura Y;et al
• 通讯作者: et al

Cluster analysis of indicators of liver functional and preoperative low branched-chain amino acid tyrosine ration indicate a high risk of early recurrence in analysis of 165 hepatocellular carcinoma patients after initial hepatectomy

• DOI: 10.1016/j.surg.2011.06.001
• 发表时间: 2011-08-01
• 期刊: SURGERY
• 影响因子: 3.8
• 作者: Nakamura, Yukio;Mizuguchi, Toru;Hirata, Koichi
• 通讯作者: Hirata, Koichi

Preoperative hepatocyte transplantation improves the survival of rats with non -alcoholic steatohepatitis-related cirrhosis after partial hepatectomy

术前肝细胞移植可提高非酒精性脂肪性肝炎相关性肝硬化大鼠部分肝切除术后的生存率

• DOI: 10.3727/096368913x668649
• 发表时间: 2013
• 期刊: Cell Transplant
• 影响因子: 3.3
• 作者: Nakamura Y;Mizuguchi T;Tanimizu N;Ichinohe N;Ooe H;Kawamoto M;Meguro M;Hirata K;Mitaka T.
• 通讯作者: Mitaka T.

Low-dose steroid pretreatment ameliorates the transient impairment of liver regeneration

低剂量类固醇预处理可改善短暂的肝再生损伤

• DOI: 10.3748/wjg.v18.i9.905
• 发表时间: 2012
• 期刊: World Journal of Gastroenterology
• 影响因子: 4.3
• 作者: Shibata T;et al
• 通讯作者: et al