The Cardioprotective Effect Of Interleukin-11 in Cardiac Surgery

白细胞介素11在心脏手术中的心脏保护作用

• 批准号: 23592027
• 负责人: KOUNO Hiroki
• 金额: $ 3.33万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592027/
• 关键词: IL-11; 心臓血管外科; 心筋保護効果

<Objective> We sought to evaluate the cardioprotective effect of IL-11 during prolonged hypothermic ischemia and subsequent reperfusion using a rat heart donor model. <Methods> IL-11 was administered intravenously before harvesting the rat heart. The hearts were preserved in cold buffer for 6 hours, and then mounted onto a Langendorff perfusion apparatus for reperfusion using an oxygenated solution containing IL-11. Normal saline was used instead of IL-11 in the control group. Functional recovery of the reperfused heart was observed and measured, and apoptotic cells were counted using the TUNEL staining technique. <Results> Functional recovery was significant after 6 hours of cold ischemia in the IL-11 group. The number of apoptotic cells was also significantly smaller in the IL-11 group. <Conclusion> IL-11 significantly protected the heart in this rat ischemic model. This was associated with the prevention of apoptosis of the cells exposed to the ischemia-reperfusion injury.

<Objective>我们试图评估IL-11在长时间低温缺血和随后的再灌注大鼠心脏供体模型的心脏保护作用。 <Methods>在收获大鼠心脏之前静脉内施用IL-11。将心脏在冷缓冲液中保存6小时，然后安装在Langendorff灌注装置上，使用含有IL-11的充氧溶液进行再灌注。对照组用生理盐水代替IL-11。观察和测量再灌注心脏的功能恢复，并使用TUNEL染色技术计数凋亡细胞。<Results>IL-11组在冷缺血6小时后功能恢复显著。IL-11组的凋亡细胞数量也明显较少。 <Conclusion>IL-11在该大鼠缺血模型中显著保护心脏。这与防止暴露于缺血-再灌注损伤的细胞凋亡有关。