Development of Novel Immuno-tolerance Treatment of Cardiac Transplantation by Cardiac Progenitor Cells from Explanted Heart

利用外植心脏的心脏祖细胞开发新型心脏移植免疫耐受治疗

• 批准号: 23592036
• 负责人: ARAI Sadahiko
• 金额: $ 3.33万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592036/
• 关键词: 冠動脈外科; 心臓血管外科; 幹細胞治療; 再生医療; 心移植; 先天性心疾患; 心筋幹細胞; 拒絶反応抑制

We purified cardiac progenitor cell from congenital heart disease patients and established iPS cells. We transplanted these cells into rat decellularized extracellular matrix by transcoronary injection. We observed neo genesis of cardiovascular tissue in peri-coronary area. This result showed possible application of regeneration therapy to bio artificial heart or transplantation. We examined gene expression of iPS cells from hypoplastic left heart syndrome patients. We observed suppression of specific basic transcription factors and increased expression of other gene group. Those gene are potentially considered to be related to expression of congenital anomaly.

我们从先天性心脏病患者中分离纯化心脏祖细胞，建立iPS细胞。我们通过冠状动脉注射将这些细胞移植到大鼠脱细胞的细胞外基质中。观察冠状动脉周围区心血管组织的新生。这一结果为再生治疗在生物人工心脏或移植中的应用提供了可能。我们检测了来自左心发育不良综合征患者的iPS细胞的基因表达。我们观察到特定的基本转录因子的抑制和其他基因组的表达增加。这些基因被认为可能与先天性畸形的表达有关。