Development of Novel Immuno-tolerance Treatment of Cardiac Transplantation by Cardiac Progenitor Cells from Explanted Heart

我们从先天性心脏病患者中分离纯化了心脏祖细胞，并建立了iPS细胞。我们通过冠状动脉注射将这些细胞移植到去细胞的大鼠细胞外基质中。观察冠脉周围区心血管组织新生血管的形成情况。这一结果表明再生疗法有可能应用于生物人工心脏或移植。我们检测了左心发育不全综合征患者iPS细胞的基因表达。我们观察到特定的基本转录因子被抑制，而其他基因组的表达增加。这些基因可能与先天畸形的表达有关。

We purified cardiac progenitor cell from congenital heart disease patients and established iPS cells. We transplanted these cells into rat decellularized extracellular matrix by transcoronary injection. We observed neo genesis of cardiovascular tissue in peri-coronary area. This result showed possible application of regeneration therapy to bio artificial heart or transplantation. We examined gene expression of iPS cells from hypoplastic left heart syndrome patients. We observed suppression of specific basic transcription factors and increased expression of other gene group. Those gene are potentially considered to be related to expression of congenital anomaly.