In Vivo and In Vitro Feasibility Studies of Intraocular Use of FocalSeal to Close Retinal Breaks

FocalSeal（FS）是一种可吸收的聚乙二醇基合成水凝胶密封剂。该液体在可见氙照明下聚合，并形成透明、柔性和牢固粘附的水凝胶。在本研究中，我们评价了密封剂的眼内生物相容性，并探讨了其关闭视网膜裂孔的有效性，并评价了FS关闭微切口玻璃体切除术的巩膜切口的能力。FS与视网膜的粘附力较强。含有FS的BSS的pH在7.2至8.0之间。眼部及组织学检查未见炎性反应。实验组和对照组的ERG无显著性差异。在我们的体外实验中，FS似乎可以有效地封闭视网膜裂孔，体内研究表明FS对眼睛没有毒性。

FocalSeal (FS) is an absorbable polyethylene glycol-based synthetic hydrogel sealant. This liquid is polymerized under visible xenon illumination, and forms clear, flexible, and firmly adherent hydrogel. In this study, we evaluated the intraocular biocompatibility of the sealant and explored its efficacy for closing retinal breaks, and evaluated the ability of FS to close sclerotomies of microincision vitrectomy.Dutch pigmented normal rabbits and enucleated porcine eyes were used in this study. Adhesion of FS to the retina was relatively strong. The pH of BSS containing FS was between 7.2 to 8.0. No inflammatory reaction was observed in the eyes by ophthalmic and histological examination. There was no significant difference in the ERG between the study and control groups. FS appeared to effectively seal retinal breaks in our in vitro experiment, and the in vivo studies indicated that FS was not toxic to the eye.