In Vivo and In Vitro Feasibility Studies of Intraocular Use of FocalSeal to Close Retinal Breaks

FocalSeal （FS）是一种可吸收的聚乙二醇基合成水凝胶密封胶。该液体在可见氙灯照射下聚合，形成清晰、柔韧、粘附牢固的水凝胶。在本研究中，我们评估了密封胶的眼内生物相容性，探讨了其对视网膜断裂闭合的功效，并评估了FS对微切口玻璃体切除术巩膜切开术的闭合能力。本研究采用荷兰色正常兔眼和去核猪眼。FS与视网膜粘连较强。含FS的BSS的pH值在7.2 ~ 8.0之间。眼科及组织学检查均未见眼部炎症反应。实验组与对照组的ERG无显著差异。在我们的体外实验中，FS似乎可以有效地密封视网膜断裂，体内研究表明FS对眼睛没有毒性。

FocalSeal (FS) is an absorbable polyethylene glycol-based synthetic hydrogel sealant. This liquid is polymerized under visible xenon illumination, and forms clear, flexible, and firmly adherent hydrogel. In this study, we evaluated the intraocular biocompatibility of the sealant and explored its efficacy for closing retinal breaks, and evaluated the ability of FS to close sclerotomies of microincision vitrectomy.Dutch pigmented normal rabbits and enucleated porcine eyes were used in this study. Adhesion of FS to the retina was relatively strong. The pH of BSS containing FS was between 7.2 to 8.0. No inflammatory reaction was observed in the eyes by ophthalmic and histological examination. There was no significant difference in the ERG between the study and control groups. FS appeared to effectively seal retinal breaks in our in vitro experiment, and the in vivo studies indicated that FS was not toxic to the eye.