Differentiation of iPS cells into salivary glands

iPS 细胞分化为唾液腺

• 批准号: 23592718
• 负责人: MISHIMA KENJI
• 金额: $ 3.24万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592718/
• 关键词: 唾液分泌障害; 再生医療; iPS細胞; 唾液腺

Salivary gland hypofunction causes not only various oral diseases but also a decrease in quality of life by systemic diseases, such as aspiration pneumonia. However, to date, there is no efficient method to treat the patients with severe symptoms. The purpose of this study is to apply a regenerative medicine using induced pluripotent (iPS) cells for these patients. We established stromal cells from salivary glands of mice at embryonic day 11.5 and 13.5, respectively. iPS cells were differentiated into oral epithelium using LCA (large-cell aggregate)-SFEBq method. These iPS cells were cultured on the established feeder cells and specific gene expressions for salivary glands were detected by RT-PCR. Consequently, gene expressions of Aqp5, M3AchR, and beta2AdR, which are highly expressed in salivary glands, were increased in these iPS cells. Therefore, these results suggested that the present method could be applied for the differentiation of iPS cells into salivary gland cells.

唾液腺功能低下不仅会导致各种口腔疾病，还会导致吸入性肺炎等全身性疾病导致生活质量下降。然而，迄今为止，还没有有效的方法来治疗症状严重的患者。本研究的目的是利用诱导多能（iPS）细胞为这些患者应用再生医学。我们分别从胚胎第 11.5 天和 13.5 天的小鼠唾液腺中建立了基质细胞。使用LCA（大细胞聚集体）-SFEBq方法将iPS细胞分化为口腔上皮。这些 iPS 细胞在建立的饲养细胞上培养，并通过 RT-PCR 检测唾液腺的特异性基因表达。因此，在唾液腺中高表达的 Aqp5、M3AchR 和 beta2AdR 的基因表达在这些 iPS 细胞中增加。因此，这些结果表明本方法可用于 iPS 细胞向唾液腺细胞的分化。

项目成果

昭和大学歯学部口腔病理

昭和大学牙科学院口腔病理学系

マウス唾液腺細胞におけるCD133陽性細胞の機能解

小鼠唾液腺细胞CD133阳性细胞的功能分析

• 发表时间: 2014
• 作者: 田中準一;安原理佳;入江太朗;秋山知恵;河野葉子;美島健二
• 通讯作者: 美島健二

マウス唾液腺細胞におけるCD133陽性細胞の機能解析

小鼠唾液腺细胞CD133阳性细胞的功能分析

• 发表时间: 2014
• 作者: 田中準一;安原理佳;入江太朗;深田俊幸;福島美和子;河野葉子;美島健二
• 通讯作者: 美島健二

Identification of gene expression profile of neural crest-derived cells isolated from submandibular glands of adult mic

成年小鼠颌下腺分离的神经嵴来源细胞基因表达谱的鉴定

• 发表时间: 2014
• 期刊: BBRC
• 作者: Takahashi M;Suzawa T;Yamada A;Yamaguchi T;Mishima K;Osumi N;Maki K;Kamijo R
• 通讯作者: Kamijo R

Identification of gene expression profile of neural crest-derived cells isolated from submandibular glands of adult mice.

鉴定从成年小鼠下颌下腺分离的神经嵴衍生细胞的基因表达谱。

• DOI: 10.1016/j.bbrc.2014.02.130
• 发表时间: 2014
• 期刊: BBRC
• 作者: Takahashi M;Suzawa T;Yamada A;Yamaguchi T;Mishima K;Osumi N;Maki K;Kamijo R
• 通讯作者: Kamijo R