Elucidation of the pathological mechanism on abnormal iron metabolism using a rat model

根据我们的发现，Sp6功能不仅在形态分化，而且在牙齿发育过程中的铁代谢，我进行了一项研究，以澄清铁代谢的作用，在Sp6的发育控制。结果表明，Sp6 Tg大鼠成釉细胞铁供应与正常对照组相同，提示有两种可能：一是细胞内溶酶体铁蛋白和铁复合物释放不完善，二是成釉细胞铁分泌不完善。然后，为了进一步分析Sp6与溶酶体功能或分泌机制的关系，我尝试用本实验室建立的牙上皮细胞构建Sp6的泰特诱导体系。不幸的是，我现在还不能领导建立泰特诱导系统。此外，我发现Sp6参与了Rock1基因的调控，这与分泌机制有关。

Based on our finding that Sp6 functions in not only morphological differentiation but also iron metabolism during tooth development, I performed a study to clarify a role of iron metabolism in the developmental control of Sp6. The iron supply for the ameloblast was the same as normal control in Sp6 Tg rat, suggesting two possibilities of the release imperfection from lysosomal ferritin and iron complex in the cell, or the secretion imperfection from the ameloblasts. Then, to further analyze the relationship between Sp6 and lysosomal function or secreting mechanism, I tried to condtruct the tet induction system for Sp6 using the dental epithelial cells that were originally established in my laboratory. Unfortunately, I could not lead to establish the tet inducible system by this moment. In addition, I found the involvement of Sp6 in Rock1 gene regulation, which is related to secretion mechanism.