Elucidation of the pathological mechanism on abnormal iron metabolism using a rat model

基于我们的发现，Sp6不仅在牙齿发育的形态分化中发挥作用，而且在牙齿发育过程中也参与铁代谢，我进行了一项研究，以阐明铁代谢在Sp6发育控制中的作用。Sp6TG大鼠成釉细胞的铁供应与正常对照组相同，提示溶酶体铁蛋白和铁复合体在细胞内释放缺陷或成釉细胞分泌缺陷两种可能性。然后，为了进一步分析Sp6与溶酶体功能或分泌机制的关系，我尝试用我实验室建立的牙齿上皮细胞构建Sp6的TET诱导系统。不幸的是，到目前为止，我还不能领导建立TET诱导性系统。此外，我还发现Sp6参与了ROCK1基因的调控，这与分泌机制有关。

Based on our finding that Sp6 functions in not only morphological differentiation but also iron metabolism during tooth development, I performed a study to clarify a role of iron metabolism in the developmental control of Sp6. The iron supply for the ameloblast was the same as normal control in Sp6 Tg rat, suggesting two possibilities of the release imperfection from lysosomal ferritin and iron complex in the cell, or the secretion imperfection from the ameloblasts. Then, to further analyze the relationship between Sp6 and lysosomal function or secreting mechanism, I tried to condtruct the tet induction system for Sp6 using the dental epithelial cells that were originally established in my laboratory. Unfortunately, I could not lead to establish the tet inducible system by this moment. In addition, I found the involvement of Sp6 in Rock1 gene regulation, which is related to secretion mechanism.