Investigation on the molecular mechanism of aquaporin 5 distribution in salivary glands of Sjogren's syndrome model mice

1.测定了与水通道蛋白5共定位的蛋白质。这些蛋白在糖尿病NOD小鼠中的定位发生了变化。2.测定了3个等电点不同的水通道蛋白5，其中正常小鼠的等电点pI为6.0，糖尿病NOD小鼠的等电点pI为8.8，与未磷酸化的水通道蛋白5的等电点一致。这表明糖尿病NOD小鼠水通道蛋白5的磷酸化水平较低。

1. The protein which co-localizes with aquaporin 5 was determined. Localization of these proteins was changed in the diabetic NOD mice. 2. Three spots of aquaporin 5 with different isoelectric points were determined.The main spot in the control mouse was detected at pI 6.0, and that in the diabetic NOD mouse was at pI 8.8 which coincides with the isoelectric point of aquaporin 5 without phosphorylation. This indicated that the phosphorylation level of aquaporin 5 was lower in the diabetic NOD mouse.