Investigation on the molecular mechanism of aquaporin 5 distribution in salivary glands of Sjogren's syndrome model mice

水通道蛋白5在干燥综合征模型小鼠唾液腺分布的分子机制研究

• 批准号: 23592781
• 负责人: TOMOKO NASHIDA
• 金额: $ 3.24万
• 依托单位: The Nippon Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592781/
• 关键词: アクアポリン; シェーグレン症候群; マウス; 耳下腺; 腺房細胞; アクアポリン５; NODマウス; 局在性; 輸送タンパク質; 口腔乾燥症; 細胞内輸送; cDNAマイクロアレイ

1. The protein which co-localizes with aquaporin 5 was determined. Localization of these proteins was changed in the diabetic NOD mice. 2. Three spots of aquaporin 5 with different isoelectric points were determined.The main spot in the control mouse was detected at pI 6.0, and that in the diabetic NOD mouse was at pI 8.8 which coincides with the isoelectric point of aquaporin 5 without phosphorylation. This indicated that the phosphorylation level of aquaporin 5 was lower in the diabetic NOD mouse.

1.确定与水通道蛋白5共定位的蛋白质。这些蛋白质的定位在糖尿病NOD小鼠中发生了变化。2.水通道蛋白5有3个等电点不同的斑点，对照组的主要斑点在pI6.0，糖尿病NOD小鼠的主要斑点在pI8.8，与水通道蛋白5的等电点一致。这表明糖尿病NOD小鼠中水通道蛋白5的磷酸化水平较低。

项目成果

Chitinase expression in parotid glands of non-obese diabetic mice

• DOI: 10.1111/j.1601-0825.2012.01904.x
• 发表时间: 2012-07-01
• 期刊: ORAL DISEASES
• 影响因子: 3.8
• 作者: Fukushima, T.;Nashida, T.;Mataga, I.
• 通讯作者: Mataga, I.

Antigen-presenting cells in parotid glands contain cystatin D originating from acinar cells.

腮腺中的抗原呈递细胞含有源自腺泡细胞的半胱氨酸蛋白酶抑制剂 D。

• DOI: 10.1016/j.abb.2012.12.009
• 发表时间: 2013
• 期刊: Arch Biochem Biophys.
• 作者: Nashida T;Sato R;Haga-Tsujimura M;Yoshie S;Yoshimura K;Imai A;Shimomura H
• 通讯作者: Shimomura H

耳下腺腺房細胞におけるRab27とRab35の働き

Rab27和Rab35在腮腺腺泡细胞中的功能

• 发表时间: 2011
• 作者: 土持眞他(監修:戸塚靖則;高戸毅);今井あかね,吉江紀夫,羽下-辻村麻衣子,梨田智子,下村浩巳
• 通讯作者: 今井あかね,吉江紀夫,羽下-辻村麻衣子,梨田智子,下村浩巳

Antibacterial proteins in parotid acinar cells of non-obese diabetic mouse

非肥胖糖尿病小鼠腮腺腺泡细胞中的抗菌蛋白

• 发表时间: 2013
• 作者: Nashida T;Yoshie S;Mizuhashi F;Sato R;Imai A;and Shimomura H
• 通讯作者: and Shimomura H

Role of Rab27-GEF in parotid acinar cell exocytosis

Rab27-GEF 在腮腺腺泡细胞胞吐作用中的作用

• 发表时间: 2013
• 作者: Imai A;Ishida M;Fukuda M;Nashida T;Shimomura H
• 通讯作者: Shimomura H