A study on optimum orthodontic force using periodontal ligament fibroblasts on 3D culture system with aging

3D培养系统上牙周膜成纤维细胞随老化最佳正畸力的研究

• 批准号: 23593011
• 负责人: KANAI Takenori
• 金额: $ 3.33万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23593011/
• 关键词: 歯根膜繊維芽細胞; 三次元立体培養; 至適矯正力

The physiological hypofunction of periodontal ligament (PDL) fibroblasts were considered to be progressing with age. In this investigation, an attempt was made to determine the optimal orthodontic force according to the change of expression of cytokines related to the osteoclasts differentiation after each compressive load on PDL fibroblasts on 3D culture system with aging.From these results, though the difference of 8-OHdG which is an indicator of aging was not observed,the relative amounts of the mRNA expression of cytokines were similar to the expression tendencies between the time of other compressions and the time of 1/4 compression, but there was a higher tendency at the time of 1/4 compression. It has been suggested that when mechanical compression is added to PDL fibroblasts in an experimental system that is assumed as in vivo, and we believe it is effective to move teeth in orthodontic treatment when the tooth was pushed by a distance of a quarter of the width of PDL.

牙周膜（PDL）成纤维细胞的生理功能减退被认为是随着年龄的增长而进展。在本研究中，尝试根据在3D培养系统上的PDL成纤维细胞在每次压缩负荷后与破骨细胞分化相关的细胞因子的表达随老化的变化来确定最佳正畸力。从这些结果来看，虽然没有观察到作为老化指标的8-OHdG的差异，细胞因子mRNA表达的相对量与其他压缩时间和1/4压缩时间之间的表达趋势相似，但在1/4压缩时间存在更高的趋势。有人建议，当机械压缩被添加到PDL成纤维细胞在一个实验系统，假设在体内，我们认为这是有效的牙齿移动正畸治疗时，牙齿被推的距离的PDL的宽度的四分之一。

项目成果

An investigation of optimal orthodontic force for periodontal ligament fibroblasts

牙周膜成纤维细胞最佳正畸力的研究

• 发表时间: 2012
• 作者: Takenori Kim;Meri Haniu;Fumio Saito;Yoshiaki Sato;Junichiro Iida
• 通讯作者: Junichiro Iida

三次元立体培養ヒト歯根膜線維芽細胞を用いた最適矯正力に関する検討

三维培养人牙周膜成纤维细胞最佳正畸力研究

• 发表时间: 2014
• 期刊: 北海道歯学雑誌
• 作者: Yamada Aya;Iwamoto Tsutomu;Fukumoto Emiko;Arakaki Makiko;Miyamoto Ryoko;Sugawara Yu;Komatsu Hideji;Nakamura Takashi;Fukumoto Satoshi;Takahashi T;羽二生芽理,金壮律,佐藤嘉晃,飯田順一郎
• 通讯作者: 羽二生芽理,金壮律,佐藤嘉晃,飯田順一郎

三次元立体培養ヒト歯根膜細胞の機械的圧縮時における破骨細胞形成に関するサイトカインの発現

三维培养人牙周膜细胞机械压缩过程中破骨细胞形成相关细胞因子的表达

• 发表时间: 2013
• 作者: 羽二生芽理;金壮律;佐藤嘉晃;飯田順一郎
• 通讯作者: 飯田順一郎

An investigation of optimal orthodontic force for human periodontal ligament fibroblasts

人牙周膜成纤维细胞最佳正畸力的研究

• 发表时间: 2011
• 作者: Takenori Kim;Meri Haniu;Yoshiaki SATO;Junichiro Iida
• 通讯作者: Junichiro Iida

Expression of Cytokines after compressive load on human periodontal ligament fibroblasts

人牙周膜成纤维细胞受压后细胞因子的表达

• 发表时间: 2013
• 作者: Takenori Kim;Meri Haniu;Yoshiaki Sato;Junichiro Iida
• 通讯作者: Junichiro Iida