Establishment of transgenic musk shrews (Suncus murinus)

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In the present study, we have established embryo manipulation techniques, such as fertilized egg collection, induction of pseudopregnancy and embryo transfer in the musk shrew. Furthermore, we successfully produced a pup from transgenic embryos in the musk shrew. So far, we have not yet produced the transgenic musk shrew expressing Venus reporter gene, but those methods established in the present study lead to generate transgenic musk shrews in the near future. In addition, we have identified promoter sequence of musk shrew GnRH2 gene, which is deficient in other rodent animal models, so that we generate GnRH2-Venus transgenic musk shrew to uncover the physiological role of GnRH2 in primates including humans.