权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Neuroendocrine mechanism mediating the nutritional infertility

介导营养性不孕的神经内分泌机制

基本信息

批准号：
08456151
负责人：
MAEDA Kei-ichiro
金额：
$ 4.35万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08456151/
关键词：
luteinizing hormone blood glucose estrogen estrogen receptor A2 region of solitary truct nucleus paraventricular nucleus noradrenergic nurons ノルアドレナリン作動性神経 2-deoxyglucose glucoprication c-fos 最後野孤束核室傍核

项目摘要

Glucose availability would be one of the major factors involved in the suppressed reproductive functions under malnutrition in mammalian species. In the present study, the role of glucose availability in regulating gonadotropin-releasing hormone (GnRH)/Iuteinizing hormone (LH) release in female rats. The sensors for detecting the blood glucose level to regulate LH secretion and feeding behavior have been considered to be located in the hypothalamus and medulla oblongata in rats. The present study focussed on the localization of glucose sensors in the area postrema using Fos immunohistochemistry as a marker of neuronal activation.Peripheral administration of a glucose antagonist, 2-deoxyglucose (2DG), induced a massive expression of Fos protein in the area postrema (AP), solitary tract nucleus (NTS) and paraventricular nucleus of the hypothalamus in ovariectomized and estradiol-primed ovariectomized rats. Dual immunohistochemistry with Fos and various cell markers, such as GFAP and MAP-2, showed that Fos-expressing cells in the AP would be neuronal but not glial cells. These cell were not catecholaminergic because any Fos-like immunoreactivities in the AP were not co-localized with tyrosine hydroxy lase immunoreactivity. A part of the Fos-expressing neruons in the NTS was catecholaminergic.These results indicate that a population of non-catecholaminergic neurons in the AP could be involved in sensing low glucose availability to regulate reproductive functions as well as feeding behavior n female rats.

葡萄糖的可用性可能是哺乳动物营养不良下生殖功能受到抑制的主要因素之一。在本研究中，葡萄糖利用率在调节雌性大鼠促性腺激素释放激素 (GnRH)/黄体生成素 (LH) 释放中的作用。用于检测血糖水平以调节 LH 分泌和进食行为的传感器被认为位于大鼠的下丘脑和延髓中。本研究的重点是使用 Fos 免疫组织化学作为神经元激活标记来定位后部区域的葡萄糖传感器。外周施用葡萄糖拮抗剂 2-脱氧葡萄糖 (2DG)，诱导后部区域 (AP)、孤束核 (NTS) 和室旁核中 Fos 蛋白的大量表达。卵巢切除和雌二醇引发的卵巢切除大鼠的下丘脑。使用 Fos 和各种细胞标记物（例如 GFAP 和 MAP-2）进行的双重免疫组织化学显示，AP 中表达 Fos 的细胞是神经元细胞，而不是神经胶质细胞。这些细胞不具有儿茶酚胺能，因为 AP 中的任何 Fos 样免疫反应性均不与酪氨酸羟化酶免疫反应性共定位。 NTS 中表达 Fos 的神经元的一部分是儿茶酚胺能神经元。这些结果表明，AP 中的一群非儿茶酚胺能神经元可能参与感知低葡萄糖可用性，以调节雌性大鼠的生殖功能和进食行为。