Study of the memory of genome stress

本研究的目的是测试人体细胞中是否存在一个系统来记忆电离辐射或抗癌药物引起的基因组损伤信息。我们对DNA损伤诱导后DNA修复蛋白RAD51与染色质的结合进行了全基因组分析。我们可以在诱导DNA损伤2小时后检测到RAD51与损伤位点和亚端粒区域的结合。在DNA损伤诱导后两周，RAD51与受损位点的结合减少。我们现在正在检查其他修复因子和组蛋白在诱导DNA损伤两周后与受损部位的结合。

The purpose of this study is to test whether there is a system to memory the information of genome damage induced by ionizing radiation or anti-cancer drugs in human cells or not. We performed genome-wide analysis of the binding of DNA repair protein RAD51 to chromatin after induction of DNA damage. We could detect the binding of RAD51 to damaged sites and sub-telomeric regions at 2 hours after induction of DNA damage. The RAD51 binding to the damaged sites were reduced at two weeks after induction of DNA damage. We are now examining the binding of other repair factors and histones to the damaged sites at two weeks after induction of DNA damage.