Molecular mechanism to construct the DNA repair related higher order nuclear structures

构建DNA修复相关高阶核结构的分子机制

• 批准号: 22310036
• 负责人: TASHIRO Satoshi
• 金额: $ 11.56万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22310036/
• 关键词: 放射線被ばく; ゲノム損傷; 細胞核構造; RAD51; 構造関連蛋白質; 細胞核構造変換

Reorganization of higher order nuclear structures has been thought to play an important role in DNA repair after ionizing radiation. However, the precise mechanism of radiation-induced reorganization of nuclear architecture is still unclear. In this study, we studied the molecular mechanisms involved in the formation of radiation induced higher order nuclear structures. We found that Matrin3 (MATR3), a nuclear structure-related protein, is involved in the regulation of the intra-nuclear dynamics of DNA repair protein RAD51. We also found that MATR3 plays a role in the regulation of the recombinational repair activity. These findings suggest the involvement of nuclear architecture in the regulation of DNA repair after ionizing radiation.

细胞核高级结构的重组被认为在电离辐射后的DNA修复中起重要作用。然而，辐射诱导的核结构重组的确切机制仍不清楚。在这项研究中，我们研究了辐射诱导的高级核结构的形成所涉及的分子机制。我们发现，Matrin3（MATR3），一个核结构相关蛋白，参与调节DNA修复蛋白RAD 51的核内动力学。我们还发现MATR 3在重组修复活性的调节中起作用。这些发现表明核结构参与电离辐射后DNA修复的调节。

项目成果

食道癌細胞株における抗癌剤感受性と DNA二重鎖切断の修復に関する検討

食管癌细胞系抗癌药物敏感性及DNA双链断裂修复研究

• 发表时间: 2010
• 作者: 青木義朗;檜原 淳;迫川賢士;恵美 学;浜井洋一;岡田守人;田代 聡
• 通讯作者: 田代 聡

p130Cas, Crk-associated substrate plays essential roles in liver development by regulating sinusoidal endothelial cell fenestration

p130Cas、Crk 相关底物通过调节肝窦内皮细胞开窗在肝脏发育中发挥重要作用

• 发表时间: 2010
• 期刊: Hepatology
• 影响因子: 13.5
• 作者: Tazaki T;Sasaki T;Uto K;Yamasaki N;Tashiro S;Sakai R;Tanaka M;Oda H;Honda Z;Honda H
• 通讯作者: Honda H

Analysis of ionizing radiation-induced chromosome abnormalities by PNA-FISH.

通过 PNA-FISH 分析电离辐射引起的染色体异常。

• 发表时间: 2012
• 作者: Satoshi Tashiro

放射線により誘発される DNA修復蛋白質による核内高次構造体形成、福島原子力発電所事故における現地での活動報告

辐射诱导DNA修复蛋白形成核高阶结构，福岛核电站事故现场活动报告

• 发表时间: 2011
• 作者: Taira N.;et al;田代 聡
• 通讯作者: 田代 聡

Bach2 represses plasma cell gene regulatory network in B cells to promote antibody class switch

• DOI: 10.1038/emboj.2010.257
• 发表时间: 2010-12-01
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Muto, Akihiko;Ochiai, Kyoko;Igarashi, Kazuhiko
• 通讯作者: Igarashi, Kazuhiko