Cytogenetically analysis of congenital abnormality and childhood malignancy using multicolor FISH methods

使用多色 FISH 方法对先天性异常和儿童恶性肿瘤进行细胞遗传学分析

• 批准号: 13670808
• 负责人: TASHIRO Satoshi
• 金额: $ 2.3万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670808/
• 关键词: Color banding FISH method; childhood leukemia

We established Color banding FISH method for the precise cytogenetical analysis, and started collection of clinical sample in 2001.We are testing the ways to transfer clinical sample to Prof. Michael Speeches, a collaborator, for multicolor FISH analysis of these clinical samples in Munich. Clinical samples of childhood leukemia are provided from "Japan Association of Childhood Leukemia Study, J*****.To identify new genes associated with childhood leukemia by the new method combining color banding FISH method and conventional FISH method to detect a specific gene, we have done functional analysis of some candidate genes. Among them, we have revealed that BACH2, a B cell specific transcription regulator, showed induction of apoptosis upon oxidative stress. Furthermore, we also found that Bach2 is expressed not only in B cell but also in brain.We have established a method to study the dynamics of chromosome in living cell to study the mechanism of chromosome translocation with Prof. Thomas Cremer, a Collaborator in Munich, and study the dynamics of chromosome territories using method.

我们建立了彩色显带荧光原位杂交（Color banding FISH）方法，用于精确的细胞遗传学分析，并于2001年开始收集临床样本。我们正在测试如何将临床样本转移给合作者Michael Speeches教授，以便在慕尼黑对这些临床样本进行荧光原位杂交（FISH）分析。本研究利用日本儿童白血病研究会（J.J.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.A.其中，我们已经揭示了BACH 2，一种B细胞特异性转录调节因子，在氧化应激时显示出凋亡诱导。此外，我们还发现Bach 2不仅在B细胞中表达，而且在脑中也有表达。我们与慕尼黑的合作者Thomas Cremer教授建立了一种研究活细胞中染色体动态的方法来研究染色体易位的机制，并利用该方法研究了染色体区域的动态。

项目成果

Muto A.: "Activation of Maf/AP-1 Repressor Bach2 by Oxidative Stress Promotes Apoptosis and Its Interaction with Promyelocytic Leukemia Nuclear Bodies"J Biol Chem. 277. 20724-20733 (2002)

Muto A.：“氧化应激激活 Maf/AP-1 阻遏蛋白 Bach2 促进细胞凋亡及其与早幼粒细胞白血病核体的相互作用”J Biol Chem。

Leach, K.: "Reconstitution of human b-Globin locus control region hypersensitive sites in the absence of chromatin assembly"Mol. Cell. Biol.. 21. 2629-2640 (2001)

Leach, K.：“在没有染色质组装的情况下重建人 b-珠蛋白基因座控制区过敏位点”Mol。

Muto A., Tashiro S., Tsuchiya H., Kume A., Kanno M., Ito E., Yamamoto M., Iarashi K.: "Activation of Maf / AP-1 repressor Bach2 by oxidative transcription factor Bach 2 in differentiating neuronal cells"J Biochem. 277(23). 427-431 (2002)

Muto A.、Tashiro S.、Tsuchiya H.、Kume A.、Kanno M.、Ito E.、Yamamoto M.、Iarashi K.：“氧化转录因子 Bach 2 在分化过程中激活 Maf / AP-1 阻遏物 Bach2

Sun, J.: "Promoter of Mouse Transcription Repressor bach1 Is Regulated by Sp1 and Trans-Activated by Bach1"J. Biochem.. 130. 385-392 (2001)

Sun, J.：“小鼠转录抑制子 bach1 的启动子受 Sp1 调节并由 Bach1 反式激活”J.

Sun J., Hoshino H., Takaku K., Nakajima O., Muto A., Suzuki H., Tashiro S., Takahashi S., Shibahara S., Alam J., Taketo MM. Yamamoto M., lgarashi K.: "Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene"EMBO J.. 21(19). 5126-5224 (2

Sun J.、Hoshino H.、Takaku K.、Nakajima O.、Muto A.、Suzuki H.、Tashiro S.、Takahashi S.、Shibahara S.、Alam J.、Taketo MM。