Cytogenetically analysis of congenital abnormality and childhood malignancy using multicolor FISH methods

我们建立了用于精确细胞遗传学分析的彩色显带FISH方法，并于2001年开始收集临床样本。我们正在测试将临床样本转移到慕尼黑的合作者Michael Speech教授进行多色FISH分析的方法。儿童白血病的临床样本由日本儿童白血病研究协会提供。为了寻找与儿童白血病相关的新基因，我们采用了色带FISH方法和常规FISH方法相结合的方法来检测特定的基因，对一些候选基因进行了功能分析。其中，我们发现B细胞特异性转录调控因子BACH2在氧化应激条件下具有诱导细胞凋亡的作用。此外，我们还发现Bach2不仅在B细胞中表达，而且在脑中也有表达。我们建立了一种研究活细胞中染色体动态的方法，与慕尼黑的合作者Thomas Cremer教授一起研究了染色体易位的机制，并利用该方法研究了染色体区域的动态。

We established Color banding FISH method for the precise cytogenetical analysis, and started collection of clinical sample in 2001.We are testing the ways to transfer clinical sample to Prof. Michael Speeches, a collaborator, for multicolor FISH analysis of these clinical samples in Munich. Clinical samples of childhood leukemia are provided from "Japan Association of Childhood Leukemia Study, J*****.To identify new genes associated with childhood leukemia by the new method combining color banding FISH method and conventional FISH method to detect a specific gene, we have done functional analysis of some candidate genes. Among them, we have revealed that BACH2, a B cell specific transcription regulator, showed induction of apoptosis upon oxidative stress. Furthermore, we also found that Bach2 is expressed not only in B cell but also in brain.We have established a method to study the dynamics of chromosome in living cell to study the mechanism of chromosome translocation with Prof. Thomas Cremer, a Collaborator in Munich, and study the dynamics of chromosome territories using method.