Development of high-performance microchip for DNA amplification towards sigle-cell genome analysis

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We successfully immobilized thermostable DNA polymerase on seven types of mesoporous silicas with different pore diameters and retained enzymatic activity sufficient for amplifying target DNA. The mesoporous silica support enabled the simple, selective, and preferential immobilization of DNA polymerase and the regulation of enzymatic activity for DNA amplification from small amounts of substrate DNA by optimizing pore size. In addition, the microfluidic chip containing composites of the enzyme and mesoporous silica exhibited precise, efficient, and continuous amplification of target DNA. Thus, these data show the great potential of mesoporous silica as enzymatic reaction platforms not only for conventional DNA amplification but also for efficient DNA amplification from small amounts of sample such as several molecules of DNA.