Live-imaging probe for strain-selective labeling of bacteria

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We have developed an approach to display various molecules on the bacterial surface using a chemically synthesized cell-wall (peptidoglycan) precursor derivative. In this research, we focused on strain-specific modification of the bacterial surface. E. coli O157 has an uncommon perosamine moiety in their O-antigen polysaccharide. We chose a precursor of perosamine, as a base for selective surface modification of E. coli O157. Six bacterial strains including E. coli O157 were incubated in growth media containing the analog, and the azido groups on the bacterial surface were labeled with a fluorophore. Flow cytometry analysis showed the selective labeling of E. coli O157.