Development of T7 promoter controlled by DNA triplex formation

由 DNA 三链体形成控制的 T7 启动子的开发

• 批准号: 23655154
• 负责人: SEIO Kohji
• 金额: $ 2.5万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655154/
• 关键词: 三重鎖核酸; 人工核酸; T7-RNAポリメラーゼ; 転写制御; 人工遺伝子

We introduce a pyrimidopyrimidoindole nucleobase(PPI) and pseudouridine as the analogs of cytosine and thymidine, respectively, in T7 promoter sequence. The artificial DNA was subjected to the transcription by usint T7-RNA polymerase. As the results, it was proved that the modified T7 promoter possessed the ability to promote transcription by T7-RNA polymerase. Moreover, it was also proved that the transctiption was inhibited by the addition of triplex forming oligonucleotide capable of recognizing the modified promoter sequence

我们在T7启动子序列中分别引入了一个嘧啶嘧啶吲哚核碱基（PPI）和假尿嘧啶作为胞嘧啶和胸腺嘧啶的类似物。人工DNA经T7-RNA聚合酶转录。结果证明，修饰后的T7启动子具有促进T7- rna聚合酶转录的能力。此外，还证明了加入能够识别修饰的启动子序列的三联体形成寡核苷酸可以抑制转录

项目成果

RNA polymerase transcription initiated by chemically modified promoters containing artificial nucleobases and its inhibition by triplex-forming oligodeoxynucleotides

由含有人工核碱基的化学修饰启动子启动的RNA聚合酶转录及其通过三链体形成寡脱氧核苷酸的抑制

• 发表时间: 2012
• 作者: Kanamori;T.; Tsunoda;H.; Ohkubo;A.; Sekine;M.; Seio;K
• 通讯作者: K

T7 RNA polymerase transcription initiated by chemically modified promoters containing artificial nucleobases and its inhibition by triplex-forming oligodeoxynucleotides

由含有人工核碱基的化学修饰启动子启动的 T7 RNA 聚合酶转录及其通过三链体形成寡脱氧核苷酸的抑制

• 发表时间: 2011
• 作者: Takashi Kanamori;Hirosuke Tsunoda;Akihiro Ohkubo;Mitsuo Sekine;Kohji Seio
• 通讯作者: Kohji Seio

DNA二重鎖および三重鎖に導入したデオキシシュードイソシチジンの塩基対形成能の評価

引入 DNA 双链体和三链体的脱氧假异胞苷的碱基配对能力评估

• 发表时间: 2012
• 作者: 山口慧;et al.
• 通讯作者: et al.

Synthesis and property of oligodeoxynucleotides doubly modified with fluorescent 2-aminopurine and a fused cytosine analog

荧光2-氨基嘌呤和融合胞嘧啶类似物双重修饰的寡脱氧核苷酸的合成和性质

• 发表时间: 2012
• 作者: Seio;K.; Tokugawa;M.; Ohzeki;H.; Tsunoda;T.; Ohkubo;A.; Sekine;M
• 通讯作者: M