Development of T7 promoter controlled by DNA triplex formation

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We introduce a pyrimidopyrimidoindole nucleobase(PPI) and pseudouridine as the analogs of cytosine and thymidine, respectively, in T7 promoter sequence. The artificial DNA was subjected to the transcription by usint T7-RNA polymerase. As the results, it was proved that the modified T7 promoter possessed the ability to promote transcription by T7-RNA polymerase. Moreover, it was also proved that the transctiption was inhibited by the addition of triplex forming oligonucleotide capable of recognizing the modified promoter sequence