Development of miRNA detection techniques using artificial nucleic acids capable of short RNA selective ginding

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In this project, we developed new techniques for miRNA detection by using organic chemistry and nucleic acid detection platforms. We tried to improve the properties of an anrtificial nucleic acid, dAChcmp, by the chemical modificaton. We also evaluated the usefulness of the new artificial nucleic as the primers for RT-PCR. We also developed new fluorescent nucleic acids as the fluorescent probes for miRNA detection. Finally, we tried to apply these new artificial nucleic acids for miRNA detection technoques such as DNA microarray.