Integration of a treatment with a device and regenerative medicine . an treatment of brain aneurysm with platinum coils is presented as an example -

有报道称，包裹生长因子等蛋白质的栓塞线圈能有效防止栓塞动脉瘤再通。然而，这些线圈很难消毒，而且不稳定，因此，不容易被批准。该项目的目标是通过开发一种新型的脑动脉瘤治疗设备，将一种治疗设备与再生医学结合起来，该设备结合了传统的栓塞线圈和低分子药物，继药物洗脱支架的成功案例之后。本项目选择治疗高胆固醇血症的他汀类药物作为低分子量药物。尝试在线圈表面形成磷脂层，将染色剂偶联到这些层中，并从偶联层中连续释放他汀类药物。利用荧光显微镜、表面等离子体共振、表面等离子体场增强荧光和细胞培养进行分析的可行性也进行了评估。从这些评价中获得了他汀类药物从磷脂层缀合和持续释放的有益信息，并组成了含有他汀类药物缀合磷脂层的线圈。这些线圈被认为有很高的潜力作为动脉瘤栓塞线圈。

It has been reported that embolization coils coated with some kinds of proteins, such as growth factors, are effective in the prevention of recanalization in embolized aneurysms. These coils are, however, difficult to be sterilized and unstable, therefore, not assumed to be approved easily. The aim of this project is integration of a treatment with a device and regenerative medicine via the development of a novel device for a therapy of brain aneurysms which combined conventional embolization coils and low-molecular-weight drugs, following a succeeded example, drug eluting stent. In this project, statins, which were drugs for the treatment of hypercholesterolemia, were selected as low-molecular-weight drugs. Formation of phospholipid layers onto coil surfaces, conjugation of stains into these layers and continuous release of statins from the conjugated layer were tried. The feasibility of analyses using a fluorescent microscope, surface plasmon resonance, surface plasmon field-enhanced fluorescent and cell culture were also assessed. Beneficial information for conjugation and continuous release of statins from the phospholipid layer for was obtained from these assessments and coils containing phospholipid layer conjugated with statins were composed. These coils were expected to have a high potential as embolization coils for aneurysms.