Integration of a treatment with a device and regenerative medicine . an treatment of brain aneurysm with platinum coils is presented as an example -

治疗与设备和再生医学的整合。

• 批准号: 23791594
• 负责人: TODA Mitsuaki
• 金额: $ 2.58万
• 依托单位: Kansai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791594/
• 关键词: 生体材料; 移植・再生医療; 血管内治療デバイス; 脳動脈瘤; 生理活性物質表面担持; 血管内治療用デバイス

It has been reported that embolization coils coated with some kinds of proteins, such as growth factors, are effective in the prevention of recanalization in embolized aneurysms. These coils are, however, difficult to be sterilized and unstable, therefore, not assumed to be approved easily. The aim of this project is integration of a treatment with a device and regenerative medicine via the development of a novel device for a therapy of brain aneurysms which combined conventional embolization coils and low-molecular-weight drugs, following a succeeded example, drug eluting stent. In this project, statins, which were drugs for the treatment of hypercholesterolemia, were selected as low-molecular-weight drugs. Formation of phospholipid layers onto coil surfaces, conjugation of stains into these layers and continuous release of statins from the conjugated layer were tried. The feasibility of analyses using a fluorescent microscope, surface plasmon resonance, surface plasmon field-enhanced fluorescent and cell culture were also assessed. Beneficial information for conjugation and continuous release of statins from the phospholipid layer for was obtained from these assessments and coils containing phospholipid layer conjugated with statins were composed. These coils were expected to have a high potential as embolization coils for aneurysms.

有报道称，包裹生长因子等蛋白质的栓塞线圈能有效防止栓塞动脉瘤再通。然而，这些线圈很难消毒，而且不稳定，因此，不容易被批准。该项目的目标是通过开发一种新型的脑动脉瘤治疗设备，将一种治疗设备与再生医学结合起来，该设备结合了传统的栓塞线圈和低分子药物，继药物洗脱支架的成功案例之后。本项目选择治疗高胆固醇血症的他汀类药物作为低分子量药物。尝试在线圈表面形成磷脂层，将染色剂偶联到这些层中，并从偶联层中连续释放他汀类药物。利用荧光显微镜、表面等离子体共振、表面等离子体场增强荧光和细胞培养进行分析的可行性也进行了评估。从这些评价中获得了他汀类药物从磷脂层缀合和持续释放的有益信息，并组成了含有他汀类药物缀合磷脂层的线圈。这些线圈被认为有很高的潜力作为动脉瘤栓塞线圈。

项目成果

集光光路を用いた表面プラズモン励起蛍光装置によるα-フェトプロテインの高感度計測の試み

使用聚焦光路的表面等离子体激发荧光装置尝试高灵敏度测量甲胎蛋白

• 发表时间: 2011
• 作者: 榎本由貴子;吉村紳一;江頭裕介;石黒光紀;宮居雅文;澤田重信;岩間 亨;戸田 満秋 他;旭 雄士;Enomoto Y;戸田 満秋 他
• 通讯作者: 戸田 満秋 他

Prototype of a surface plasmon field-enhanced fluorescence spectroscopy apparatus with a condensed optical system

具有聚光光学系统的表面等离子体场增强荧光光谱仪原型

• 发表时间: 2012
• 作者: TODA Mitsuaki;et. al.
• 通讯作者: et. al.

Quantitative Measurement of Alpha-Fetoprotein and Prostate Specific Antigen using Surface Plasmon Field Enhanced Fluorescence Spectroscopy

使用表面等离子体场增强荧光光谱法定量测定甲胎蛋白和前列腺特异性抗原

• 发表时间: 2011
• 作者: TODA Mitsuaki;et. al.
• 通讯作者: et. al.

表面プラズモン場励起蛍光法を用いたバイオマーカーの高感度検出の試み

表面等离子体场激发荧光法高灵敏检测生物标志物的尝试

• 发表时间: 2011
• 作者: 榎本由貴子;吉村紳一;江頭裕介;石黒光紀;宮居雅文;澤田重信;岩間 亨;戸田 満秋 他
• 通讯作者: 戸田 満秋 他

集光光路を用いた表面プラズモン励起蛍光装置によるα－フェトプロテインの高感度計測の試み

使用聚焦光路的表面等离子体激发荧光装置尝试高灵敏度测量甲胎蛋白

• 发表时间: 2011
• 作者: 戸田 満秋;有馬 祐介;岩田 博夫
• 通讯作者: 岩田 博夫