The mechanisms of induction of autophagic cell death by histone deacetylase inhibitor SAHA

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In electron microscope SAHA induced autophagosome in multidrug-resistant osteosarcoma cell. SAHA also induced autophagy related protein Beclin1 and LC3-I 、II in dose dependent manner. These facts demonstrated that SAHA induce autophagic cell death in multidrug-resistant osteosarcoma cell. Next, we investigated cell cycle population in osteosarcoma parental cell and multidrug-resistant cell after SAHA treatment. SAHA increased Sub-G1 fraction in parental cell, but G2/M fraction in multidrug-resistant cell. Therefore, we investigated the relationship between autopphagy and G2/M phase. As a result, we got new fact that SAHA induce autophagy and G2/M phase at the same time