The integrated search of mesenchymal stem cell fate determination of differentiation controlled by micro RNAs

Micro RNA调控间充质干细胞分化命运决定的综合研究

• 批准号: 23791657
• 负责人: MIZUNO Yosuke
• 金额: $ 2.66万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791657/
• 关键词: 骨・軟骨代謝学; 非コードRNA; 転写制御; 細胞分化; マイクロRNA

In this study, we identified a microRNA which are transcriptionally regulated by the transcription factor osterix that have critical roles for osteoblast differentiation. We identified that this micro RNA targets the genes which promote osteoblast differentiation and inhibit their function, and transcription of this micro RNA itself is negatively regulated by osterix. Moreover, we also found that this micro RNA negatively regulates osteoblast differentiation. Therefore, in this study we found a micro RNA that could regulate the fate of mesenchymal stem cells for osteoblast differentiation. This finding further contributes the understanding of pathogenic mechanisms with micro RNAs to the life-style diseases such as osteoporosis and obesity.

在这项研究中，我们发现了一个受转录因子Osterix转录调控的microRNA，它对成骨细胞的分化具有关键作用。我们发现这种微小的RNA以促进成骨细胞分化和抑制其功能的基因为靶标，并且这种微小的RNA本身的转录受到Osterix的负调控。此外，我们还发现，这种微小的RNA负向调节成骨细胞的分化。因此，在本研究中，我们发现了一种能够调控间充质干细胞向成骨细胞分化的微RNA。这一发现进一步有助于理解微RNA在骨质疏松症和肥胖症等生活型疾病中的致病机制。

项目成果

間葉系幹細胞の分化を制御する転写因子によるマイクロRNAの調節

控制间充质干细胞分化的转录因子对 microRNA 的调节

• 作者: 水野 洋介;仲地 豊;徳澤 佳美;八塚由紀子 ;岡崎 康司
• 通讯作者: 岡崎 康司

Regulation of differentiation via microRNAs controlled by master regulator transcription factors during adipocyte/osteoblast differentiation

在脂肪细胞/成骨细胞分化过程中，通过主调控转录因子控制的 microRNA 来调节分化

• 发表时间: 2012
• 作者: Yosuke Mizuno;Yutaka Nakachi;Yoshimi Tokuzawa;Yukiko Yatsuka;Yasushi Okazaki
• 通讯作者: Yasushi Okazaki

Sirt1, p53, and p38MAPK are Crucial Regulators of Detrimental Phenotypes of Embryonic Stem Cells with Max Expression Ablation

• DOI: 10.1002/stem.1147
• 发表时间: 2012-08-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Hishida, Tomoaki;Nozaki, Yuriko;Okuda, Akihiko
• 通讯作者: Okuda, Akihiko

Identification of Ccr4-Not Complex Components as Regulators of Transition from Partial to Genuine Induced Pluripotent Stem Cells

• DOI: 10.1089/scd.2013.0326
• 发表时间: 2014-09-15
• 期刊: STEM CELLS AND DEVELOPMENT
• 影响因子: 4
• 作者: Kamon, Masayoshi;Katano, Miyuki;Okuda, Akihiko
• 通讯作者: Okuda, Akihiko

Regulation of Adipocyte/Osteoblast Differentiation by microRNAs That Are Controlled by Master Regulatory Transcription Factors

由主调控转录因子控制的 microRNA 对脂肪细胞/成骨细胞分化的调节

• 发表时间: 2013
• 作者: Yosuke Mizuno;Yutaka Nakachi;Yoshimi Tokuzawa;Yukiko Kanesaki-Yatsuka;Yasushi Okazaki
• 通讯作者: Yasushi Okazaki