The elucidation of the MRTF mediated molecular mechanism of renalinterstitial fibrosis in obstructive nephropathy

我们研究了MRTF siRNA抑制MRTF对大鼠肾间质成纤维细胞系（NRK-49 F）中肾间质成纤维细胞活化的影响，以及对单侧输尿管梗阻（UUO）诱导的肾纤维化小鼠模型中纤维化发展的影响。在体外，我们证实了MRTF的功能意义的成纤维细胞表型的维护。然而，在UUO小鼠模型中，我们还没有证实MRTF siRNA可以减少肾间质纤维化。由于MRTF siRNA的有效性还不够，我们无法获得确切的结果。最近，我们一直在研究MRTF siRNA转染的合适条件。

We examined the effect of MRTF inhibition by MRTF siRNAs on the activation of renal interstitial fibroblasts in a rat renal interstitial fibroblast line (NRK -49F) and on the development of fibrosis in a mouse model of renal fibrosis induced by unilateral ureteral obstruction (UUO). In vitro, we confirmed the functional significance of MRTF for the maintenance of fibroblast phenotype. However, in a mouse model of UUO, we have not confirmed that MRTF siRNAs can decrease renal interstitial fibrosis yet. Because the effectiveness of MRTF siRNAs is not sufficient, we can not obtain the exact results. Recently, we have been examining the right conditions of MRTF siRNAs transfections.