the development of synthetic microRNA to cancer stem cell of gynecological cancer

合成microRNA对妇科癌症干细胞的开发

• 批准号: 23791860
• 负责人: TSUDA Naotake
• 金额: $ 2.83万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791860/
• 关键词: 婦人科腫瘍学; 癌幹細胞; 上皮間葉転換; 子宮体癌; microRNA; 卵巣癌

Recently, the number of patients with endometrial cancer has been rapidly growing in Japan. In this cancer, endometrioid adneocarcinoma gradeIII have especially poor diagnosis. The present study was undertaken to examine the mechanism of the resistance to chemotherapy for endometrial cancer and "salinomycin" which is the treatment drug for cancer stem cell. We speculate that there would be resistant cancer stem cells to "salynomycin", as evidenced by MTT assay which showed incomplete growth repression for "salinomycin" to endometrial cancer cell line. Therefore, we constructed "salynomycin" resistant cell line. And we examined the expression rate of ALDH(cancer stem cell marker) by flowcytometer, and cancer stemness gene expression by Real time PCR. In our study, the expression rate of ALDH(high) was significantly observed in "salinomycin" resistant cell line. Also, we found extensively high expression of L1CAM(L1 cell adhesion molecule) by Real time PCR.

最近，日本子宫内膜癌患者的数量一直在迅速增长。在这种癌症中，子宫内膜类药物肾上腺素瘤级别的诊断尤其差。本研究进行了研究，以检查对子宫内膜癌和“盐霉素”化学疗法的耐药性的机制，这是癌症干细胞的治疗药物。我们推测将有抗性癌细胞对“丙霉素”的抗性干细胞，如MTT分析所证明的那样，显示“盐霉素”对子宫内膜癌细胞系的生长不完全抑制。因此，我们构建了“丙霉素”耐药细胞系。我们通过流动仪检查了ALDH（癌干细胞标记）的表达速率，并通过实时PCR检查了癌症干基因表达。在我们的研究中，在“盐霉素”耐药细胞系中显着观察到ALDH（高）的表达速率。同样，我们通过实时PCR发现了L1CAM（L1细胞粘附分子）的广泛高表达。