Roles of soluble VEGF receptor on angiogenesis of eye in vivo

在这里，我们显示了调节可溶性Flt-1（一种潜在的抗血管生成因子）的替代3 '端加工的机制。我们发现hnRNP D通过与Flt-1前体mRNA内含子13上的调控序列结合而下调可溶性Flt-1的表达。在此基础上，我们利用慢病毒介导的精原干细胞转基因技术进行了研究。提示慢病毒颗粒不能感染精原干细胞，故该方法无效。因此，我们将通过常规方法建立转基因小鼠，并在体内研究可溶性Flt-1对眼部血管生成的作用。

Here, we showed the mechanisms for regulating alternative 3'-end processing of soluble Flt-1, a potential antiangiogenic factor. We found that hnRNP D down-regulated soluble Flt-1 expression by binding to the regulatory sequence on intron 13 of Flt-1 pre-mRNA. Furthermore, we carried out the lentiviral mediated transgenesis by in vivo manipulation of spermatogonical stem cell. It was suggested that the method was not effective because lentiviral particle couldn't infect to spermatogonical stem cell. Therefore, we are going to create a transgenic mouse conventionally and show the roles of soluble Flt-1 on angiogenesis of eye in vivo.