Effect of the treatment for the anal canal dysfunction using dedifferentiated fat cell(DFAT)

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I found significantlyα-Smooth muscle actin (αSMA) positive cell at the smooth muscle differentiation medium compared with the control (5%FCS), cultured rat DFAT with the smooth muscle differentiation medium (5%FCS, TGFβ5ng/ml). To confirm whether rat DFAT have possibility of smooth muscle and skeletal muscle differentiation in vivo, I producted anal canal (it consists of smooth muscle and skeletal muscle) dysfunction rat model using cardiotoxin (CTX). Green fluorescence protein (GFP)-labeled DFAT or PBS were injected into para-anal canal tissue. Immunohistochemistry revealed that inner smooth muscle and external striated muscle layers in anal canal showed atrophy or necrosis in the control group, whereas DFAT transplantation led an increase of smoothe muscle and striated muscle mass with variable fiber orientation. GFP-labeled DFAT model showed a decrease of inflammatory cells in HE stain. DFAT cell transplantation resulted in a significant improvement of anal canal pressure, compared with control. DFAT cell transplantation promotes anal canal muscle regeneration and improves anal canal pressure.